Cadmium-inducible metallothionein in Tilapia (Oreochromis mossambicus)

Mature tilapia Oreochromis mossambicus were exposed to water contaminated with cadmium at concentrations of 6 7 mg/l for 13 14 d, or injected with 0.2 mg cadmium chloride/kg every 2 d for a total of three injections, and metallothionein was studied. In the injected fish, liver contained the highest...

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Bibliographic Details
Published in:Bulletin of environmental contamination and toxicology 1999-06, Vol.62 (6), p.758-768
Main Authors: WU, S. M, WENG, C. F, YU, M. J, LIN, C. C, CHEN, S. T, HWANG, J. C, HWANG, P. P
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Cadmium
Cadmium Chloride - pharmacokinetics
Cadmium Chloride - toxicity
Carcinogens - pharmacokinetics
Carcinogens - toxicity
Chromatography, High Pressure Liquid
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Metallothionein - biosynthesis
Oreochromis mossambicus
Tilapia - metabolism
Tissue Distribution
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Summary:Mature tilapia Oreochromis mossambicus were exposed to water contaminated with cadmium at concentrations of 6 7 mg/l for 13 14 d, or injected with 0.2 mg cadmium chloride/kg every 2 d for a total of three injections, and metallothionein was studied. In the injected fish, liver contained the highest Cd concentrations, followed by intestine and kidney, while in water-exposed fish, Cd levels were highest in intestine, followed by liver and kidney. Compared with the nucleic acid sequence of tilapia metallothionein cDNA, the data showed over 90% similarity between amino acid and nucleic acid sequences. Results showed that Cd-inducible metallothionein was confirmed by purification, characterization, and sequencing.
ISSN:0007-4861
1432-0800
DOI:10.1007/s001289900937