Two-dimensional crystallization of herpes simplex virus type 1 single-stranded DNA-binding protein, ICP8, on a lipid monolayer

• Published in: Biochimica et biophysica acta 2004-09, Vol.1701 (1), p.101-108
• Main Authors: Makhov, Alexander M., Taylor, Dianne W., Griffith, Jack D.
• Format: Article
• Language: English
• Subjects: Crystallization; Crystallography, X-Ray; Crystals; DNA-Binding Proteins; Herpes simplex virus 1; Herpes simplex virus type 1; ICP8; Lipid monolayer; Microscopy, Electron; Negative staining; SSB; Transmission electron microscopy; Viral Proteins - chemistry
• ISSN: 1570-9639; 0006-3002; 1878-1454
• DOI: 10.1016/j.bbapap.2004.06.006

Herpes simplex virus type 1 single-stranded DNA-binding protein (ICP8) has been crystallized on a positively charged lipid monolayer. The crystals belong to the planar group p2 with a=39 nm, b=23.2 nm and γ=87.2°. The projected map of ICP8 crystals calculated at a resolution of 3.9 nm shows four ICP8 monomers per unit cell with the crystals formed by a parallel arrangement of 16.2 nm helical ICP8 filaments. This novel filamentous form has not been reported before. The ICP8 monomers show different appearances in projection, suggesting that they may adopt different orientations, probably reflecting the strong intermolecular and lipid–filament interactions in the crystal. When the 23 nm diameter filaments formed by ICP8 in solution at low temperature in the presence of magnesium were generated and then layered on the phospholipid monolayer, highly ordered arrays of an 8.5 nm filament with a shallow 31.2 nm pitch were observed and reconstruction revealed a double-helical structure.