Standard Operating Procedure for Immunuslotblot Assay for Analysis of DNA/Sulfur Mustard Adducts in Human Blood and Skin

A standard operating procedure has been developed for an immunoslotblot assay of sulfur mustard adducts to DNA in human blood and skin for use in a field laboratory. A minimum detectable level of exposure of human blood in vitro (≥ 50nM) sulfur mustard is feasible with the assay. In the case of huma...

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Bibliographic Details
Published in:Journal of analytical toxicology 2004-07, Vol.28 (5), p.316-319
Main Authors: van der Schans, G.P., Mars-Groenendijk, R., de Jong, L.P.A., Benschop, H.P., Noort, D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Chemical Warfare Agents - chemistry
Chemical Warfare Agents - poisoning
DNA Adducts - analysis
DNA Adducts - chemistry
Gas, fumes
Humans
Immunoblotting - standards
Leukocytes - chemistry
Medical sciences
Military Medicine - methods
Mustard Gas - chemistry
Mustard Gas - poisoning
Reference Values
Skin - chemistry
Skin - drug effects
Toxicology
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Summary:A standard operating procedure has been developed for an immunoslotblot assay of sulfur mustard adducts to DNA in human blood and skin for use in a field laboratory. A minimum detectable level of exposure of human blood in vitro (≥ 50nM) sulfur mustard is feasible with the assay. In the case of human skin, a 1 s exposure to saturated sulfur mustard vapor (830 mg/m−3) could still be detected.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/28.5.316