ANABAENA AND MICROCYSTIS IN THE PACIFIC NORTHWEST: A TALE OF PLASTICITY

The molecular marker, cpcBA‐IGS, was used to examine the biogeography of representatives of Anabaena and Microcystis collected from Pacific Northwest lakes and their phylogenetic relationship to isolates from the UTEX and CCAP culture collections. cpcBA‐IGS contains portions of the alpha and beta su...

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Bibliographic Details
Published in:Journal of phycology 2000-12, Vol.36 (s3), p.71-71
Main Authors: Williams, C.R., Peters, B., Waaland, J.R.
Format: Article
Language:English
Subjects:
Anabaena
Cyanophyta
Freshwater
Microcystis
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Summary:The molecular marker, cpcBA‐IGS, was used to examine the biogeography of representatives of Anabaena and Microcystis collected from Pacific Northwest lakes and their phylogenetic relationship to isolates from the UTEX and CCAP culture collections. cpcBA‐IGS contains portions of the alpha and beta subunit of cyanophycocyanin with an intergenic spacer (IGS) of ∼100 base pairs situated between the subunits. The use of this cyanobacteria specific marker and primers allowed us to amplify cpcBA‐IGS DNA from uni‐cyanobacterial field samples. The morphology and habit of specimens were documented by photomicroscopy. Single filaments or colonies of field material and 3–4 filaments of culture material were then amplified using the polymerase chain reaction (PCR). Results include analysis of non‐toxic summer and toxic winter blooms of Anabaena from 1989–1997 in American Lake, WA and a comparison of non‐toxic and toxic Microcystis from Lake Sammamish, WA. A preliminary phylogenetics analysis of Pacific Northwest and UTEX isolates also was performed using parsimony, maximum likelihood and distance methods. The results of this analysis suggest that revision of Anabaena and Microcystis taxonomy may be needed.
ISSN:0022-3646
1529-8817
DOI:10.1046/j.1529-8817.1999.00001-210.x