Light-Inducible MiR163 Targets PXMT1 Transcripts to Promote Seed Germination and Primary Root Elongation in Arabidopsis

• Published in: Plant physiology (Bethesda) 2016-03, Vol.170 (3), p.1772-1782
• Main Authors: Chung, Pil Joong, Park, Bong Soo, Wang, Huan, Liu, Jun, Jang, In-Cheol, Chua, Nam-Hai
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Gene Expression Profiling - methods; Gene Expression Regulation, Developmental - radiation effects; Gene Expression Regulation, Plant - radiation effects; Germination - genetics; Light; Methyltransferases - genetics; Methyltransferases - metabolism; MicroRNAs - genetics; Mutation; Plant Roots - genetics; Plant Roots - growth & development; Plant Roots - metabolism; Plants, Genetically Modified; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Seedlings - genetics; Seedlings - growth & development; Seedlings - metabolism; Seeds - genetics; Seeds - growth & development; Seeds - metabolism; SIGNALING AND RESPONSE
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.15.01188

Expression of many plant microRNAs is responsive to hormones and environmental stimuli, but none has yet been associated with light. Arabidopsis (Arabidopsis thaliana) miR163 is 24 nucleotides in length and targets mRNAs encoding several S-adenosyl-Metdependent carboxyl methyltransferase family members. Here, we found that miR163 is highly induced by light during seedling deetiolation as well as seed germination. Under the same condition, its target PXMT1, encoding a methyltransferase that methylates 1,7-paraxanthine, is down-regulated. Light repression of PXMT1 is abolished in a mir163 null mutant, but the repression can be restored to wild-type levels in complementation lines expressing pri-miR163 gene in the mir163 mutant background. During seed germination, miR163 and its target PXMT1 are predominantly expressed in the radicle, and the expression patterns of the two genes are inversely correlated. Moreover, compared with the wild type, mir163 mutant or PXMT1 overexpression line shows delayed seed germination under continuous light, and seedlings develop shorter primary roots with an increased number of lateral roots under long-day condition. Together, our results indicate that miR163 targets PXMT1 mRNA to promote seed germination and modulate root architecture during early development of Arabidopsis seedlings.