Amyloplast Membrane Protein SUBSTANDARD STARCH GRAIN6 Controls Starch Grain Size in Rice Endosperm

• Published in: Plant physiology (Bethesda) 2016-03, Vol.170 (3), p.1445-1459
• Main Authors: Matsushima, Ryo, Maekawa, Masahiko, Kusano, Miyako, Tomita, Katsura, Kondo, Hideki, Nishimura, Hideki, Crofts, Naoko, Fujita, Naoko, Sakamoto, Wataru
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; CELL BIOLOGY; Cytoplasmic Granules - genetics; Cytoplasmic Granules - metabolism; Endosperm - genetics; Endosperm - metabolism; Gene Expression Regulation, Plant; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Immunoblotting; Intracellular Membranes - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Microscopy, Confocal; Microscopy, Electron, Transmission; Mutation; Oligonucleotide Array Sequence Analysis; Oryza - genetics; Oryza - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plastids - genetics; Plastids - metabolism; Plastids - ultrastructure; Pollen - genetics; Pollen - metabolism; Sequence Homology, Amino Acid; Starch - metabolism; Transaminases - genetics; Transaminases - metabolism
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.15.01811

Starch is a biologically and commercially important polymer of glucose. Starch is organized into starch grains (SGs) inside amyloplasts. The SG size differs depending on the plant species and is one of the most important factors for industrial applications of starch. There is limited information on genetic factors regulating SG sizes. In this study, we report the rice (Oryza sativa) mutant substandard starch grain6 (ssg6), which develops enlarged SGs in endosperm. Enlarged SGs are observed starting at 3 d after flowering. During endosperm development, a number of smaller SGs appear and coexist with enlarged SGs in the same cells. The ssg6 mutation also affects SG morphologies in pollen. The SSG6 gene was identified by map-based cloning and microarray analysis. SSG6 encodes a protein homologous to aminotransferase. SSG6 differs from other rice homologs in that it has a transmembrane domain. SSG6-green fluorescent protein is localized in the amyloplast membrane surrounding SGs in rice endosperm, pollen, and pericarp. The results of this study suggest that SSG6 is a novel protein that controls SG size. SSG6 will be a useful molecular tool for future starch breeding and applications.