New Analytical Tool for the Detection of Ractopamine Abuse in Goat Skeletal Muscle by Potential Gene Expression Biomarkers

• Published in: Journal of agricultural and food chemistry 2016-03, Vol.64 (8), p.1861-1867
• Main Authors: Zhao, Luyao, Yang, Shuming, Zhang, Yanhua, Zhang, Ying, Hou, Can, Cheng, Yongyou, You, Xinyong, Gu, Xu, Zhao, Zhen, Muhammad Tarique, Tunio
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers - metabolism; Gene Expression - drug effects; Goats - genetics; Goats - metabolism; Growth Substances - administration & dosage; Growth Substances - metabolism; Muscle, Skeletal - drug effects; Muscle, Skeletal - growth & development; Muscle, Skeletal - metabolism; Phenethylamines - administration & dosage; Phenethylamines - metabolism
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.5b04956

In this study, quantification of mRNA gene expression was examined as biomarkers to detect ractopamaine abuse and ractopamaine residues in cashmere goats. It was focused on the identification of potential gene expression biomarkers and describing the coreletionship between gene expression and residue level by 58 animals for 49 days. The results showed that administration periods and residue levels significantly influenced mRNA expressions of the β2-adrenergic receptor (β2AR), the enzymes PRKACB, ADCY3, ATP1A3, ATP2A3, PTH, and MYLK, and the immune factors IL-1β and TNF-α. Statistical analysis like principal components analysis (PCA), hierarchical cluster analysis (HCA), and discriminant analysis (DA) showed that these genes can serve as potential biomarkers for ractopamine in skeletal muscle and that they are also suitable for describing different residue levels separately.