Nanogold bioconjugates for direct and sensitive multiplexed immunosensing

The use of nanogold bioconjugates for direct detection of the antibody–antigen immunoreaction is addressed. The integration of gold nanoparticles tracers as signal generators in microarray immunosensing and compact disc detection technique show important advantages to reach sensitive, selective, hig...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-07, Vol.69, p.294-300
Main Authors: Dobosz, P., Morais, S., Puchades, R., Maquieira, A.
Format: Article
Language:English
Subjects:
Assaying
Compact Disks
Complex Mixtures - analysis
Density
Equipment Design
Equipment Failure Analysis
Gold
Gold - chemistry
Immunoassay
Immunoassay - instrumentation
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Microarray Analysis - instrumentation
Microarraying
Multiplex
Multiplexing
Nano-gold
Nanoconjugates - chemistry
Nanoconjugates - ultrastructure
Nanoparticles
Nanostructure
Recognition
Tracers
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Summary:The use of nanogold bioconjugates for direct detection of the antibody–antigen immunoreaction is addressed. The integration of gold nanoparticles tracers as signal generators in microarray immunosensing and compact disc detection technique show important advantages to reach sensitive, selective, high throughput, reliable and cost-effective assays. For that, a thorough study of the performances of the size of spherical nanogold particles and coating density was developed. The size of the nanoparticle determines the optimal antibody dilution, being the smaller particles the best performing ones. Enhancement effect of lower size is also studied. The gold labeling method do not affects the recognition capability of the labeled proteins. As a proof of concept, the nanoconjugates were used for the simultaneous and direct determination of small molecules. Employing nanogold bioconjugates as recognition labels resulted in robust and reliable assays, reaching a sensitivity of 0.03 and 1.3μg/L for sulfasalazine and atrazine, respectively. This shows that the use of nanogold bioconjugates for direct immunosensing is very competitive, achieving highly sensitive and reproducible assays (RSD
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2015.03.007