Expression of enzymes for the usage in food and feed industry with Pichia pastoris

•We compare the Expression system Pichia pastoris to other established systems in protein production.•We summarise the state-of-the-art of protein production using Pichia pastoris.•Give detailed information on enzymes used in food and feed production and show ways of alternative production with Pich...

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Bibliographic Details
Published in:Journal of biotechnology 2015-05, Vol.202, p.118-134
Main Authors: Spohner, Sebastian C., Müller, Hagen, Quitmann, Hendrich, Czermak, Peter
Format: Article
Language:English
Subjects:
Biotechnology
Density
Enzymes
Enzymes - biosynthesis
Enzymes - genetics
Fermentation
Food and feed industry
Food Industry
Foods
Fructooligosaccharides
Phytase
Pichia - genetics
Pichia - growth & development
Pichia pastoris
Protein expression
Proteins
Recombinant
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Yeast
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Summary:•We compare the Expression system Pichia pastoris to other established systems in protein production.•We summarise the state-of-the-art of protein production using Pichia pastoris.•Give detailed information on enzymes used in food and feed production and show ways of alternative production with Pichia pastoris. The methylotrophic yeast Pichia pastoris is an established protein expression host for the production of industrial enzymes. This yeast can be grown to very high cell densities and produces high titers of recombinant protein, which can be expressed intercellularly or be secreted to the fermentation medium. P. pastoris offers some advantages over other established expression systems especially in protein maturation. In food and feed production many enzymatically catalyzed processes are reported and the demand for new enzymes grows continuously. For instance the unique catalytic properties of enzymes are used to improve resource efficiency, maintain quality, functionalize food, and to prevent off-flavors. This review aims to provide an overview on recent developments in heterologous production of enzymes with P. pastoris and their application within the food sector.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2015.01.027