Expression of enzymes for the usage in food and feed industry with Pichia pastoris

• Published in: Journal of biotechnology 2015-05, Vol.202, p.118-134
• Main Authors: Spohner, Sebastian C., Müller, Hagen, Quitmann, Hendrich, Czermak, Peter
• Format: Article
• Language: English
• Subjects: Biotechnology; Density; Enzymes; Enzymes - biosynthesis; Enzymes - genetics; Fermentation; Food and feed industry; Food Industry; Foods; Fructooligosaccharides; Phytase; Pichia - genetics; Pichia - growth & development; Pichia pastoris; Protein expression; Proteins; Recombinant; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Yeast
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2015.01.027

•We compare the Expression system Pichia pastoris to other established systems in protein production.•We summarise the state-of-the-art of protein production using Pichia pastoris.•Give detailed information on enzymes used in food and feed production and show ways of alternative production with Pichia pastoris. The methylotrophic yeast Pichia pastoris is an established protein expression host for the production of industrial enzymes. This yeast can be grown to very high cell densities and produces high titers of recombinant protein, which can be expressed intercellularly or be secreted to the fermentation medium. P. pastoris offers some advantages over other established expression systems especially in protein maturation. In food and feed production many enzymatically catalyzed processes are reported and the demand for new enzymes grows continuously. For instance the unique catalytic properties of enzymes are used to improve resource efficiency, maintain quality, functionalize food, and to prevent off-flavors. This review aims to provide an overview on recent developments in heterologous production of enzymes with P. pastoris and their application within the food sector.