An ultrasensitive electrochemical DNA biosensor based on graphene/Au nanorod/polythionine for human papillomavirus DNA detection

An ultrasensitive electrochemical DNA biosensor for human papillomavirus (HPV) detection was developed by electrochemical impedance spectroscopy and differential pulse voltammetry. A capture probe was immobilized on a glassy carbon electrode modified with graphene/Au nanorod/polythionine (G/Au NR/PT...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-06, Vol.68, p.442-446
Main Authors: Huang, Huayu, Bai, Wanqiao, Dong, Changxun, Guo, Rui, Liu, Zhihua
Format: Article
Language:English
Subjects:
Biosensing Techniques
Biosensors
Deoxyribonucleic acid
DNA, Viral - chemistry
DNA, Viral - genetics
DNA, Viral - isolation & purification
Electrochemical Techniques
Electrodes
Gold
Gold - chemistry
Graphene
Graphite - chemistry
Human
Human papillomavirus
Humans
Nanostructure
Nanotubes - chemistry
Papillomaviridae - isolation & purification
Papillomaviridae - pathogenicity
Phenothiazines - chemistry
Self assembly
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Summary:An ultrasensitive electrochemical DNA biosensor for human papillomavirus (HPV) detection was developed by electrochemical impedance spectroscopy and differential pulse voltammetry. A capture probe was immobilized on a glassy carbon electrode modified with graphene/Au nanorod/polythionine (G/Au NR/PT). Two auxiliary probes were designed and used to long-range self-assemble DNA nanostructure. The target DNA can connect DNA structure to the capture probe on the electrode surface. [Ru(phen)3](2+) was selected as a redox indicator for amplifying electrochemical signal significantly. Enhanced sensitivity was obtained through combining the excellent electric conductivity of G/Au NR/PT architecture and the long-range self-assembly DNA nanostructure with the multi-signal amplification. The DNA biosensor displayed excellent performance for HPV DNA detection over the range from 1.0×10(-13) to 1.0×10(-10) mol/L with a detection limit of 4.03×10(-14) mol/L. Furthermore, the proposed method can also be used for the detection of HPV DNA in human serum samples and provides a potential application of DNA detection in clinic research.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2015.01.039