Bio-inspired oligovitronectin-grafted surface for enhanced self-renewal and long-term maintenance of human pluripotent stem cells under feeder-free conditions

Abstract Current protocols for human pluripotent stem cell (hPSC) expansion require feeder cells or matrices from animal sources that have been the major obstacle to obtain clinical grade hPSCs due to safety issues, difficulty in quality control, and high expense. Thus, feeder-free, chemically defin...

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Bibliographic Details
Published in:Biomaterials 2015-05, Vol.50, p.127-139
Main Authors: Park, Hyun-Ji, Yang, Kisuk, Kim, Mun-Jung, Jang, Jiho, Lee, Mihyun, Kim, Dong-Wook, Lee, Haeshin, Cho, Seung-Woo
Format: Article
Language:English
Subjects:
Adhesion
Advanced Basic Science
Amino Acid Sequence
Biomimetic Materials - pharmacology
Cell Communication - drug effects
Cell Proliferation - drug effects
Cell Self Renewal - drug effects
Cells, Cultured
Clone Cells
Culture
Dentistry
Embryo, Mammalian - cytology
Embryo, Mammalian - drug effects
Feeder Cells - cytology
Feeder Cells - drug effects
Feeder-free maintenance
Focal Adhesions - drug effects
Human
Human pluripotent stem cells
Humans
Indoles - chemistry
Maintenance
Material-independent surface coating
Molecular Sequence Data
Obstacles
PDA
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - drug effects
Polydopamine
Polymers - chemistry
Quality
Signal Transduction - drug effects
Stem cells
Surface Properties
Vitronectin - chemistry
Vitronectin - pharmacology
Vitronectin peptide
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Description
Summary:Abstract Current protocols for human pluripotent stem cell (hPSC) expansion require feeder cells or matrices from animal sources that have been the major obstacle to obtain clinical grade hPSCs due to safety issues, difficulty in quality control, and high expense. Thus, feeder-free, chemically defined synthetic platforms have been developed, but are mostly confined to typical polystyrene culture plates. Here, we report a chemically defined, material-independent, bio-inspired surface coating allowing for feeder-free expansion and maintenance of self-renewal and pluripotency of hPSCs on various polymer substrates and devices. Polydopamine (pDA)-mediated immobilization of vitronectin (VN) peptides results in surface functionalization of VN-dimer/pDA conjugates. The engineered surfaces facilitate adhesion, proliferation, and colony formation of hPSCs via enhanced focal adhesion, cell–cell interaction, and biophysical signals, providing a chemically defined, xeno-free culture system for clonal expansion and long-term maintenance of hPSCs. This surface engineering enables the application of clinically-relevant hPSCs to a variety of biomedical systems such as tissue-engineering scaffolds and medical devices.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2015.01.015