Collagen/chitosan based two-compartment and bi-functional dermal scaffolds for skin regeneration

• Published in: Materials Science & Engineering C 2015-07, Vol.52, p.155-162
• Main Authors: Wang, Feng, Wang, Mingbo, She, Zhending, Fan, Kunwu, Xu, Cheng, Chu, Bin, Chen, Changsheng, Shi, Shengjun, Tan, Rongwei
• Format: Article
• Language: English
• Subjects: Animals; Antibacterial activity; Antiinfectives and antibacterials; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biomimetic dermal scaffold; Cell Line; Cell Proliferation - drug effects; Cell Survival - drug effects; Chitosan; Collagen; Collagens; Drug delivery; Encapsulation; Fibroblasts - cytology; Fibroblasts - drug effects; Humans; Mice; Microspheres; Polyglycolic Acid - chemistry; Porosity; Regeneration; rhVEGF; Scaffolds; Serratia marcescens - drug effects; Skin regeneration; Skin, Artificial; Staphylococcus aureus - drug effects; Tissue Engineering - methods; Tissue Scaffolds - chemistry; Vascular Endothelial Growth Factor A - chemistry
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2015.03.013

Inspired from the sophisticated bilayer structures of natural dermis, here, we reported collagen/chitosan based two-compartment and bi-functional dermal scaffolds. Two functions refer to mediating rapid angiogenesis based on recombinant human vascular endothelial growth factor (rhVEGF) and antibacterial from gentamicin, which were encapsulated in PLGA microspheres. The gentamicin and rhVEGF encapsulated PLGA microspheres were further combined with collagen/chitosan mixtures in low (lower layer) and high (upper layer) concentrations, and molded to generate the two-compartment and bi-functional scaffolds. Based on morphology and pore structure analyses, it was found that the scaffold has a distinct double layered porous and connective structure with PLGA microspheres encapsulated. Statistical analysis indicated that the pores in the upper layer and in the lower layer have great variations in diameter, indicative of a two-compartment structure. The release profiles of gentamicin and rhVEGF exceeded 28 and 49days, respectively. In vitro culture of mouse fibroblasts showed that the scaffold can facilitate cell adhesion and proliferation. Moreover, the scaffold can obviously inhibit proliferation of Staphylococcus aureus and Serratia marcescens, exhibiting its unique antibacterial effect. The two-compartment and bi-functional dermal scaffolds can be a promising candidate for skin regeneration. •The dermal scaffold is inspired from the bilayer structures of natural dermis.•The dermal scaffold has two-compartment structures.•The dermal scaffold containing VEGF and gentamicin encapsulated PLGA microspheres•The dermal scaffold can facilitate cell adhesion and proliferation.