Athrombogenic hydrogel coatings for medical devices – Examination of biological properties

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2015-06, Vol.130, p.192-198
• Main Authors: Butruk-Raszeja, Beata A., Łojszczyk, Ilona, Ciach, Tomasz, Kościelniak-Ziemniak, Magdalena, Janiczak, Karolina, Kustosz, Roman, Gonsior, Małgorzata
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Biocompatibility; Blood; Blood compatibility; Blood Platelets - chemistry; Blood Platelets - drug effects; Blood Platelets - metabolism; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Coatings; Density; Durability; Fibrinogen; Fibrinogen - chemistry; Humans; Hydrogel; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; Hydrogels; Materials Testing; Models, Animal; Platelet; Platelet Adhesiveness - drug effects; Platelets; Polyurethane; Polyurethanes - chemistry; Povidone - chemistry; Prostheses and Implants; Rabbits; Reduction; Surface modification; Surface Properties
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2015.04.008

•Hydrogel coatings for improving hemocompatibility of polyurethanes was proposed.•Excellent durability – no significant changes in coating thickness over 35-day period.•Biopassive properties – twice the reduction in surface adsorption of serum-derived fibrinogen.•Biocompatibility – no toxic effects of the implanted material on the surrounding animal tissues. In the article the authors present hydrogel coatings prepared from polyvinylpyrrolidone (PVP) macromolecules, which are chemically bonded to polyurethane (PU) substrate. The coating is designed to improve the surface hemocompatibility of blood-contacting medical devices. The coating was characterized in terms of physical properties (swelling ratio, hydrogel density, surface morphology, coating thickness, coating durability). In order to examine surface hemocompatibility, the materials were contacted with whole human blood under arterial flow simulated conditions followed by calculation of platelet consumption and the number of platelet aggregates. Samples were also contacted with platelet-poor plasma; the number of surface-adsorbed fibrinogen molecules was measured using ELISA assay. Finally, the inflammatory reaction after implantation was assessed, using New Zealand rabbits. The designed coating is characterized by high water content and excellent durability in aqueous environment – over a 35-day period, no significant changes in coating thickness were observed. Experiments with blood proved twice the reduction in adsorption of serum-derived fibrinogen together with a moderate reduction in the number of platelet aggregates formed during the contact of the material with blood. The analysis of an inflammatory reaction after the implantation confirmed high biocompatibility of the fabricated materials – studies have shown no toxic effects of the implanted material on the surrounding animal tissues.