Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments

High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffractio...

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Bibliographic Details
Published in:Physical review letters 2014-02, Vol.112 (8), Article 088102
Main Authors: Weinhausen, Britta, Saldanha, Oliva, Wilke, Robin N, Dammann, Christian, Priebe, Marius, Burghammer, Manfred, Sprung, Michael, Koster, Sarah
Format: Article
Language:English
Subjects:
Aqueous environments
Beams (radiation)
Cells (biology)
Diffraction
Microfluidics
Scanning
Tasks
X-rays
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Summary:High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm.
ISSN:0031-9007
1079-7114
DOI:10.1103/physrevlett.112.088102