Determination of low urinary 8-hydroxy-2`-deoxyguanosine excretion with capillary electrophoresis and molecularly imprinted monolith solid phase microextraction

• Published in: The Science of the total environment 2013-04, Vol.450-451, p.266-270
• Main Authors: Zhang, Shaowen, Song, Xia, Zhang, Weiya, Luo, Nan, Cai, Lingshuang
• Format: Article
• Language: English
• Subjects: 8-Hydroxy-2`-deoxyguanosine; Adult; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - urine; Electrophoresis; Electrophoresis, Capillary - methods; Humans; Limit of Detection; Linear Models; Middle Aged; Reproducibility of Results; Solid phase microextraction; Solid Phase Microextraction - methods; Urine; Young Adult
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2013.02.025

As a sensitive biomarker, the level of 8-hydroxy-2`-deoxyguanosine (8-OHdG) indicates the extent of DNA oxidative damage of subject. However its determination in human urine is confounded by trace content and a complex matrix. In this study, a new approach of solid phase microextraction (SPME) coupled to capillary electrophoresis and electrochemical detection was developed with molecularly imprinted monolithic column as extraction sorbent. The tailor made monolith exhibited high extraction efficiency with the enrichment factor 73 for 8-OHdG owing to its special porous structure and inherent selectivity. Under optimal conditions, appreciable sensitivity had been achieved with a limit of detection of 2.61nmol/L (signal/noise=3) and a limit of quantification of 8.63nmol/L (signal/noise=10), respectively. As an alternative of precise 8-OHdG determination, wide range linearity had been achieved with 0.01–1.50μmol/L and RSDs with 3.70% for migration time and 4.80% for peak current (10–1000nmol/L). 106 samples with relative low extents of DNA oxidative damage were collected and analyzed which including coke plant workers, traffic policemen, taxi drivers and healthy students. Elevated urinary 8-OHdG excretions of subjects may correlate to working condition, occupational exposure, or lifestyle. ► 8-Hydroxy-2`-deoxyguanosine (8-OHdG) is a sensitive biomarker of DNA oxidative damage. ► Urinary 8-OHdG determination is confounded by trace content and complex matrix. ► High extraction efficiency and selectivity were exhibited for the prepared monolith used as extraction sorbent. ► Coupled to capillary electrophoresis, a precise approach was developed for urinary 8-OHdG determination. ► 106 subjects with relative low 8-OHdG excretions were analyzed successfully.