Loading…

Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone

Abstract This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer re...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomechanics 2015-06, Vol.48 (8), p.1479-1484
Main Authors: Zellner, J, Mueller, M, Xin, Y, Krutsch, W, Brandl, A, Kujat, R, Nerlich, M, Angele, P
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823
cites cdi_FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823
container_end_page 1484
container_issue 8
container_start_page 1479
container_title Journal of biomechanics
container_volume 48
creator Zellner, J
Mueller, M
Xin, Y
Krutsch, W
Brandl, A
Kujat, R
Nerlich, M
Angele, P
description Abstract This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer region of the meniscus. Aggregates of human fibrochondrocytes with cell origin from the inner region or with cell origin from the outer region were generated. From the two groups of either cell origin, aggregates were treated with dynamic hydrostatic pressure (1 Hz for 4 h; 0.55–5.03 MPa, cyclic sinusoidal) from day 1 to day 7. The other aggregates served as unloaded controls. At day 0, 7, 14 and 21 aggregates were harvested for evaluation including histology, immunostaining and ELISA analysis for glycosaminoglycan (GAG) and collagen II. Loaded aggregates were found to be macroscopically larger and revealed immunohistochemically enhanced chondrogenesis compared to the corresponding controls. Loaded or non-loaded meniscal cells from the outer zone showed a higher potential and earlier onset of chondrogenesis compared to the cells from the inner part of the meniscus. This study suggests that intrinsic factors like cell properties in the different areas of the meniscus and their reaction on mechanical load might play important roles in designing Tissue Engineering strategies for meniscal repair in vivo.
doi_str_mv 10.1016/j.jbiomech.2015.02.003
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1770362657</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S002192901500069X</els_id><sourcerecordid>1732839011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823</originalsourceid><addsrcrecordid>eNqNkk2PFCEQQInRuOPoX9iQePEyYwHdNH0xmvUz2cSDmngjDBQOYzeM0L1m_PUyzq4me1kPhAp5VVC8IuScwZoBk893690mpBHtds2BtWvgawBxjyyY6sSKCwX3yQKAs1XPezgjj0rZAUDXdP1DcsZb2SvewIL8fH2IZgyWbg8upzKZqcb7jKXMGSnGrYkWC3XBe8wYp2CG4UCnLVK7TbGmfMOIJRSaPB0xhmLNQC0OQ6E-p_EPGWLETE10NM1TjX6liI_JA2-Ggk-u9yX58vbN54v3q8uP7z5cvLpcWcmbaYVNo9quYYjgO1Vji03rsPHWtqZv64FQ6B16yVyvmtr8xovOSaMawZziYkmeneruc_oxY5n0WN9Y32ciprlo1nUgJJdt9x-o4Er0wNjdqFQCOtmCqujTW-guzTnWno8U50KwupZEnihbJZSMXu9zGE0-aAb6KFzv9I1wfRSugesqvCaeX5efNyO6v2k3hivw8gRg_eWrgFkXG7BKdSGjnbRL4e47XtwqYYcQQzX9HQ9Y_vWjS03Qn45jd5w61taJk_1X8RskJdTh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1682233123</pqid></control><display><type>article</type><title>Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Zellner, J ; Mueller, M ; Xin, Y ; Krutsch, W ; Brandl, A ; Kujat, R ; Nerlich, M ; Angele, P</creator><creatorcontrib>Zellner, J ; Mueller, M ; Xin, Y ; Krutsch, W ; Brandl, A ; Kujat, R ; Nerlich, M ; Angele, P</creatorcontrib><description>Abstract This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer region of the meniscus. Aggregates of human fibrochondrocytes with cell origin from the inner region or with cell origin from the outer region were generated. From the two groups of either cell origin, aggregates were treated with dynamic hydrostatic pressure (1 Hz for 4 h; 0.55–5.03 MPa, cyclic sinusoidal) from day 1 to day 7. The other aggregates served as unloaded controls. At day 0, 7, 14 and 21 aggregates were harvested for evaluation including histology, immunostaining and ELISA analysis for glycosaminoglycan (GAG) and collagen II. Loaded aggregates were found to be macroscopically larger and revealed immunohistochemically enhanced chondrogenesis compared to the corresponding controls. Loaded or non-loaded meniscal cells from the outer zone showed a higher potential and earlier onset of chondrogenesis compared to the cells from the inner part of the meniscus. This study suggests that intrinsic factors like cell properties in the different areas of the meniscus and their reaction on mechanical load might play important roles in designing Tissue Engineering strategies for meniscal repair in vivo.</description><identifier>ISSN: 0021-9290</identifier><identifier>EISSN: 1873-2380</identifier><identifier>DOI: 10.1016/j.jbiomech.2015.02.003</identifier><identifier>PMID: 25698240</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Aggregates ; Biomechanics ; Bone surgery ; Cartilage, Articular - cytology ; Cell Differentiation ; Cells, Cultured ; Chondrocytes - physiology ; Chondrogenesis ; Collagen ; Collagen - metabolism ; Dynamic hydrostatic pressure ; Dynamics ; ELISA ; Extracellular matrix ; Extracellular Matrix - metabolism ; Fibrochondrocytes ; Glycosaminoglycans ; Glycosaminoglycans - metabolism ; Human ; Humans ; Hydrostatic Pressure ; Joint surgery ; Knee ; Loads (forces) ; Mechanobiology ; Menisci, Tibial - cytology ; Meniscus ; Origins ; Physical Medicine and Rehabilitation ; Stem cells ; Tissue Engineering</subject><ispartof>Journal of biomechanics, 2015-06, Vol.48 (8), p.1479-1484</ispartof><rights>Elsevier Ltd</rights><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823</citedby><cites>FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25698240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zellner, J</creatorcontrib><creatorcontrib>Mueller, M</creatorcontrib><creatorcontrib>Xin, Y</creatorcontrib><creatorcontrib>Krutsch, W</creatorcontrib><creatorcontrib>Brandl, A</creatorcontrib><creatorcontrib>Kujat, R</creatorcontrib><creatorcontrib>Nerlich, M</creatorcontrib><creatorcontrib>Angele, P</creatorcontrib><title>Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone</title><title>Journal of biomechanics</title><addtitle>J Biomech</addtitle><description>Abstract This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer region of the meniscus. Aggregates of human fibrochondrocytes with cell origin from the inner region or with cell origin from the outer region were generated. From the two groups of either cell origin, aggregates were treated with dynamic hydrostatic pressure (1 Hz for 4 h; 0.55–5.03 MPa, cyclic sinusoidal) from day 1 to day 7. The other aggregates served as unloaded controls. At day 0, 7, 14 and 21 aggregates were harvested for evaluation including histology, immunostaining and ELISA analysis for glycosaminoglycan (GAG) and collagen II. Loaded aggregates were found to be macroscopically larger and revealed immunohistochemically enhanced chondrogenesis compared to the corresponding controls. Loaded or non-loaded meniscal cells from the outer zone showed a higher potential and earlier onset of chondrogenesis compared to the cells from the inner part of the meniscus. This study suggests that intrinsic factors like cell properties in the different areas of the meniscus and their reaction on mechanical load might play important roles in designing Tissue Engineering strategies for meniscal repair in vivo.</description><subject>Aggregates</subject><subject>Biomechanics</subject><subject>Bone surgery</subject><subject>Cartilage, Articular - cytology</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Chondrocytes - physiology</subject><subject>Chondrogenesis</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Dynamic hydrostatic pressure</subject><subject>Dynamics</subject><subject>ELISA</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibrochondrocytes</subject><subject>Glycosaminoglycans</subject><subject>Glycosaminoglycans - metabolism</subject><subject>Human</subject><subject>Humans</subject><subject>Hydrostatic Pressure</subject><subject>Joint surgery</subject><subject>Knee</subject><subject>Loads (forces)</subject><subject>Mechanobiology</subject><subject>Menisci, Tibial - cytology</subject><subject>Meniscus</subject><subject>Origins</subject><subject>Physical Medicine and Rehabilitation</subject><subject>Stem cells</subject><subject>Tissue Engineering</subject><issn>0021-9290</issn><issn>1873-2380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkk2PFCEQQInRuOPoX9iQePEyYwHdNH0xmvUz2cSDmngjDBQOYzeM0L1m_PUyzq4me1kPhAp5VVC8IuScwZoBk893690mpBHtds2BtWvgawBxjyyY6sSKCwX3yQKAs1XPezgjj0rZAUDXdP1DcsZb2SvewIL8fH2IZgyWbg8upzKZqcb7jKXMGSnGrYkWC3XBe8wYp2CG4UCnLVK7TbGmfMOIJRSaPB0xhmLNQC0OQ6E-p_EPGWLETE10NM1TjX6liI_JA2-Ggk-u9yX58vbN54v3q8uP7z5cvLpcWcmbaYVNo9quYYjgO1Vji03rsPHWtqZv64FQ6B16yVyvmtr8xovOSaMawZziYkmeneruc_oxY5n0WN9Y32ciprlo1nUgJJdt9x-o4Er0wNjdqFQCOtmCqujTW-guzTnWno8U50KwupZEnihbJZSMXu9zGE0-aAb6KFzv9I1wfRSugesqvCaeX5efNyO6v2k3hivw8gRg_eWrgFkXG7BKdSGjnbRL4e47XtwqYYcQQzX9HQ9Y_vWjS03Qn45jd5w61taJk_1X8RskJdTh</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Zellner, J</creator><creator>Mueller, M</creator><creator>Xin, Y</creator><creator>Krutsch, W</creator><creator>Brandl, A</creator><creator>Kujat, R</creator><creator>Nerlich, M</creator><creator>Angele, P</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TB</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone</title><author>Zellner, J ; Mueller, M ; Xin, Y ; Krutsch, W ; Brandl, A ; Kujat, R ; Nerlich, M ; Angele, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aggregates</topic><topic>Biomechanics</topic><topic>Bone surgery</topic><topic>Cartilage, Articular - cytology</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Chondrocytes - physiology</topic><topic>Chondrogenesis</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Dynamic hydrostatic pressure</topic><topic>Dynamics</topic><topic>ELISA</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibrochondrocytes</topic><topic>Glycosaminoglycans</topic><topic>Glycosaminoglycans - metabolism</topic><topic>Human</topic><topic>Humans</topic><topic>Hydrostatic Pressure</topic><topic>Joint surgery</topic><topic>Knee</topic><topic>Loads (forces)</topic><topic>Mechanobiology</topic><topic>Menisci, Tibial - cytology</topic><topic>Meniscus</topic><topic>Origins</topic><topic>Physical Medicine and Rehabilitation</topic><topic>Stem cells</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zellner, J</creatorcontrib><creatorcontrib>Mueller, M</creatorcontrib><creatorcontrib>Xin, Y</creatorcontrib><creatorcontrib>Krutsch, W</creatorcontrib><creatorcontrib>Brandl, A</creatorcontrib><creatorcontrib>Kujat, R</creatorcontrib><creatorcontrib>Nerlich, M</creatorcontrib><creatorcontrib>Angele, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Physical Education Index</collection><collection>Health &amp; Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomechanics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zellner, J</au><au>Mueller, M</au><au>Xin, Y</au><au>Krutsch, W</au><au>Brandl, A</au><au>Kujat, R</au><au>Nerlich, M</au><au>Angele, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone</atitle><jtitle>Journal of biomechanics</jtitle><addtitle>J Biomech</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>48</volume><issue>8</issue><spage>1479</spage><epage>1484</epage><pages>1479-1484</pages><issn>0021-9290</issn><eissn>1873-2380</eissn><abstract>Abstract This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer region of the meniscus. Aggregates of human fibrochondrocytes with cell origin from the inner region or with cell origin from the outer region were generated. From the two groups of either cell origin, aggregates were treated with dynamic hydrostatic pressure (1 Hz for 4 h; 0.55–5.03 MPa, cyclic sinusoidal) from day 1 to day 7. The other aggregates served as unloaded controls. At day 0, 7, 14 and 21 aggregates were harvested for evaluation including histology, immunostaining and ELISA analysis for glycosaminoglycan (GAG) and collagen II. Loaded aggregates were found to be macroscopically larger and revealed immunohistochemically enhanced chondrogenesis compared to the corresponding controls. Loaded or non-loaded meniscal cells from the outer zone showed a higher potential and earlier onset of chondrogenesis compared to the cells from the inner part of the meniscus. This study suggests that intrinsic factors like cell properties in the different areas of the meniscus and their reaction on mechanical load might play important roles in designing Tissue Engineering strategies for meniscal repair in vivo.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>25698240</pmid><doi>10.1016/j.jbiomech.2015.02.003</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0021-9290
ispartof Journal of biomechanics, 2015-06, Vol.48 (8), p.1479-1484
issn 0021-9290
1873-2380
language eng
recordid cdi_proquest_miscellaneous_1770362657
source ScienceDirect Freedom Collection 2022-2024
subjects Aggregates
Biomechanics
Bone surgery
Cartilage, Articular - cytology
Cell Differentiation
Cells, Cultured
Chondrocytes - physiology
Chondrogenesis
Collagen
Collagen - metabolism
Dynamic hydrostatic pressure
Dynamics
ELISA
Extracellular matrix
Extracellular Matrix - metabolism
Fibrochondrocytes
Glycosaminoglycans
Glycosaminoglycans - metabolism
Human
Humans
Hydrostatic Pressure
Joint surgery
Knee
Loads (forces)
Mechanobiology
Menisci, Tibial - cytology
Meniscus
Origins
Physical Medicine and Rehabilitation
Stem cells
Tissue Engineering
title Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A04%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dynamic%20hydrostatic%20pressure%20enhances%20differentially%20the%20chondrogenesis%20of%20meniscal%20cells%20from%20the%20inner%20and%20outer%20zone&rft.jtitle=Journal%20of%20biomechanics&rft.au=Zellner,%20J&rft.date=2015-06-01&rft.volume=48&rft.issue=8&rft.spage=1479&rft.epage=1484&rft.pages=1479-1484&rft.issn=0021-9290&rft.eissn=1873-2380&rft_id=info:doi/10.1016/j.jbiomech.2015.02.003&rft_dat=%3Cproquest_cross%3E1732839011%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c624t-e4485741ee0f78485ce45de4fcc5a9548538efdef61d984187bf37d6a8431d823%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1682233123&rft_id=info:pmid/25698240&rfr_iscdi=true