Platinum(II)-Oligonucleotide Coordination Based Aptasensor for Simple and Selective Detection of Platinum Compounds

Wide use of platinum-based chemotherapeutic regimens for the treatment for carcinoma calls for a simple and selective detection of platinum compound in biological samples. On the basis of the platinum­(II)-base pair coordination, a novel type of aptameric platform for platinum detection has been int...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-10, Vol.87 (20), p.10542-10546
Main Authors: Cai, Sheng, Tian, Xueke, Sun, Lianli, Hu, Haihong, Zheng, Shirui, Jiang, Huidi, Yu, Lushan, Zeng, Su
Format: Article
Language:English
Subjects:
Activation
Analytical chemistry
Animals
Aptamers, Nucleotide - chemistry
Beads
Binding
Catalysis
Chemical compounds
Chemiluminescence
Chemotherapy
Cisplatin - urine
Horseradish Peroxidase - metabolism
Hydrogen
Luminescence
Luminescent Measurements - instrumentation
Oligonucleotides - chemistry
Organoplatinum Compounds - analysis
Organoplatinum Compounds - chemistry
Platinum
Platinum - chemistry
Platinum compounds
Rats
Rats, Sprague-Dawley
Sensors
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Summary:Wide use of platinum-based chemotherapeutic regimens for the treatment for carcinoma calls for a simple and selective detection of platinum compound in biological samples. On the basis of the platinum­(II)-base pair coordination, a novel type of aptameric platform for platinum detection has been introduced. This chemiluminescence (CL) aptasensor consists of a designed streptavidin (SA) aptamer sequence in which several base pairs were replaced by G-G mismatches. Only in the presence of platinum, coordination occurs between the platinum and G-G base pairs as opposed to the hydrogen-bonded G-C base pairs, which leads to SA aptamer sequence activation, resulting in their binding to SA coated magnetic beads. These Pt-DNA coordination events were monitored by a simple and direct luminol-peroxide CL reaction through horseradish peroxidase (HRP) catalysis with a strong chemiluminescence emission. The validated ranges of quantification were 0.12–240 μM with a limit of detection of 60 nM and selectivity over other metal ions. This assay was also successfully used in urine sample determination. It will be a promising candidate for the detection of platinum in biomedical and environmental samples.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b02810