Platinum(II)-Oligonucleotide Coordination Based Aptasensor for Simple and Selective Detection of Platinum Compounds

• Published in: Analytical chemistry (Washington) 2015-10, Vol.87 (20), p.10542-10546
• Main Authors: Cai, Sheng, Tian, Xueke, Sun, Lianli, Hu, Haihong, Zheng, Shirui, Jiang, Huidi, Yu, Lushan, Zeng, Su
• Format: Article
• Language: English
• Subjects: Activation; Analytical chemistry; Animals; Aptamers, Nucleotide - chemistry; Beads; Binding; Catalysis; Chemical compounds; Chemiluminescence; Chemotherapy; Cisplatin - urine; Horseradish Peroxidase - metabolism; Hydrogen; Luminescence; Luminescent Measurements - instrumentation; Oligonucleotides - chemistry; Organoplatinum Compounds - analysis; Organoplatinum Compounds - chemistry; Platinum; Platinum - chemistry; Platinum compounds; Rats; Rats, Sprague-Dawley; Sensors; Symbols
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.5b02810

Wide use of platinum-based chemotherapeutic regimens for the treatment for carcinoma calls for a simple and selective detection of platinum compound in biological samples. On the basis of the platinum­(II)-base pair coordination, a novel type of aptameric platform for platinum detection has been introduced. This chemiluminescence (CL) aptasensor consists of a designed streptavidin (SA) aptamer sequence in which several base pairs were replaced by G-G mismatches. Only in the presence of platinum, coordination occurs between the platinum and G-G base pairs as opposed to the hydrogen-bonded G-C base pairs, which leads to SA aptamer sequence activation, resulting in their binding to SA coated magnetic beads. These Pt-DNA coordination events were monitored by a simple and direct luminol-peroxide CL reaction through horseradish peroxidase (HRP) catalysis with a strong chemiluminescence emission. The validated ranges of quantification were 0.12–240 μM with a limit of detection of 60 nM and selectivity over other metal ions. This assay was also successfully used in urine sample determination. It will be a promising candidate for the detection of platinum in biomedical and environmental samples.