Endogenous hydrogen peroxide in the hypothalamic paraventricular nucleus regulates neurohormonal excitation in high salt-induced hypertension

• Published in: Toxicology letters 2015-06, Vol.235 (3), p.206-215
• Main Authors: Zhang, Meng, Qin, Da-Nian, Suo, Yu-Ping, Su, Qing, Li, Hong-Bao, Miao, Yu-Wang, Guo, Jing, Feng, Zhi-Peng, Qi, Jie, Gao, Hong-Li, Mu, Jian-Jun, Zhu, Guo-Qing, Kang, Yu-Ming
• Format: Article
• Language: English
• Subjects: Amitrole - pharmacology; Animals; Blood Pressure - drug effects; Catalase; Catalase - pharmacology; Cytokines; Diets; Gene Expression Regulation - drug effects; Heart Rate - drug effects; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Hypertension; Hypertension - chemically induced; Hypothalamic paraventricular nucleus; Male; Neurotransmitter Agents - blood; Neurotransmitter Agents - metabolism; Neurotransmitters; Nuclei; Paraventricular Hypothalamic Nucleus - metabolism; Polyethylene Glycols - pharmacology; Rats; Rats, Sprague-Dawley; Reactive oxygen species; Renin-angiotensin system; Sodium Chloride, Dietary - toxicity
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/j.toxlet.2015.04.008

•Salt-induced hypertensive rats exhibit neurohormonal excitation in the PVN.•PVN PEG-CAT attenuates neurohormonal excitation and expression of RAS in hypertension.•PVN PEG-CAT attenuates imbalances of neurotransmitters and cytokines in hypertension.•PVN ATZ augments hypertension-induced neurohormonal excitation and expression of RAS.•PVN ATZ augments hypertension-induced imbalances of neurotransmitters and cytokines. Reactive oxygen species (ROS) in the brain plays an important role in the progression of hypertension and hydrogen peroxide (H2O2) is a major component of ROS. The aim of this study is to explore whether endogenous H2O2 changed by polyethylene glycol-catalase (PEG-CAT) and aminotriazole (ATZ) in the hypothalamic paraventricular nucleus (PVN) regulates neurotransmitters, renin-angiotensin system (RAS), and cytokines, and whether subsequently affects the renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) in high salt-induced hypertension. Male Sprague–Dawley rats received a high-salt diet (HS, 8% NaCl) or a normal-salt diet (NS, 0.3% NaCl) for 10 weeks. Then rats were treated with bilateral PVN microinjection of PEG-CAT (0.2 i.u./50nl), an analog of endogenous catalase, the catalase inhibitor ATZ (10nmol/50nl) or vehicle. High salt-fed rats had significantly increased MAP, RSNA, plasma norepinephrine (NE) and pro-inflammatory cytokines (PICs). In addition, rats with high-salt diet had higher levels of NOX-2, NOX-4 (subunits of NAD(P)H oxidase), angiotensin-converting enzyme (ACE), interleukin-1beta (IL-1β), glutamate and NE, and lower levels of gamma-aminobutyric acid (GABA) and interleukin-10 (IL-10) in the PVN than normal diet rats. Bilateral PVN microinjection of PEG-CAT attenuated the levels of RAS and restored the balance of neurotransmitters and cytokines, while microinjection of ATZ into the PVN augmented those changes occurring in hypertensive rats. Our findings demonstrate that ROS component H2O2 in the PVN regulating MAP and RSNA are partly due to modulate neurotransmitters, renin-angiotensin system, and cytokines within the PVN in salt-induced hypertension.