Cloning and nucleotide sequence of a gene encoding a glycogen debranching enzyme in the trehalose operon from Arthrobacter sp. Q361

A gene located just upstream of the treYZ operon was isolated from Arthrobacter sp. strain Q36. The gene, designated treX, encoded an 823-amino acid protein. The amino acid sequence of the protein had 50% identity with the TreX protein (isoamylase) from Sulfolobus acidocaldarius ATCC 33909 which has...

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Bibliographic Details
Published in:Biochimica et biophysica acta, Protein structure and molecular enzymology Protein structure and molecular enzymology, 2000-02, Vol.1476 (2), p.377-381
Main Authors: Maruta, Kazuhiko, Kubota, Michio, Fukuda, Shigeharu, Kurimoto, Masashi
Format: Article
Language:English
Subjects:
Arthrobacter
glycogen debranching enzyme
Sulfolobus acidocaldarius
trehalose
treX gene
TreX protein
treXYZ operon
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Summary:A gene located just upstream of the treYZ operon was isolated from Arthrobacter sp. strain Q36. The gene, designated treX, encoded an 823-amino acid protein. The amino acid sequence of the protein had 50% identity with the TreX protein (isoamylase) from Sulfolobus acidocaldarius ATCC 33909 which has a treZXY operon on the genome. We suggest that Arthrobacter treX is an isoamylase gene, and that it is a component of a treXYZ operon.
ISSN:0167-4838