Production of cellulase by Clostridium papyrosolvens CFR-703

Clostridium papyrosolvens producing filter paperase, carboxymethyl cellulase and cellobiase under anaerobic cultivation conditions at 35 °C is described. Higher activities of filter paperase and carboxymethylcellulases were assayed in 48 h culture filtrate, while maximum cellobiase accumulated in th...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2004, Vol.20 (6), p.629-632
Main Authors: SWAROOPA RANI, D, THIRUMALE, Sharmila, NAND, Krishna
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cellulase
Cellulose
Clostridium papyrosolvens
Cultivation
Filtrate
Fundamental and applied biological sciences. Psychology
Microbiology
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Summary:Clostridium papyrosolvens producing filter paperase, carboxymethyl cellulase and cellobiase under anaerobic cultivation conditions at 35 °C is described. Higher activities of filter paperase and carboxymethylcellulases were assayed in 48 h culture filtrate, while maximum cellobiase accumulated in the culture broth at 72 h. Filter paperase, carboxymethylcellulase and cellobiase activities were optimum at 35 °C and pH values of 7.0, 6.5 and 7.5 respectively. Cultivation of the strain in 1000 ml Hungate bottles with 1% cellulose at pH 6.5 and 35 °C produced carboxymethyl cellulase, filter paperase and cellobiase activities of 45, 35 and 20 IU/ml respectively.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1023/B:WIBI.0000043193.39486.32