Influence of biological fluids in bacterial viability on different hospital surfaces and fomites

Background The hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. The present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces...

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Bibliographic Details
Published in:American journal of infection control 2016-03, Vol.44 (3), p.311-314
Main Authors: Esteves, Deigilam C., ScM, Pereira, Valeria C., PhD, Souza, Joyce M., BS, Keller, Rogéria, PhD, Simões, Rebeca D., PhD, Winkelstroter Eller, Lizziane K., PhD, Rodrigues, Marcus Vinicius P., PhD
Format: Article
Language:English
Subjects:
Bacteria
Bacteria - isolation & purification
Body fluids
Body Fluids - microbiology
Colony Count, Microbial
Contamination
Cross-infection
Environmental Microbiology
Fomites
Fomites - microbiology
Hospitals
Humans
Infection Control
Infectious Disease
Microbial Viability
Nosocomial infections
Pathogens
Time Factors
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Summary:Background The hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. The present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. Methods Four different fomites and surfaces (ceramic floor, cotton fabric fragments and synthetic fibers, and eggcrate foam mattress) were contaminated with potential pathogens ( Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa , and Klebsiella pneumoniae ), then submitted to influence of biological fluids (blood, urine, artificial saliva). The viability of strains was evaluated at 24 hours after contamination and then in intervals of 7 days, by the colony-forming unit count technique. Results S aureus presented viability (>70 days) in all conditions tested, E faecalis and K pneumoniae had decreased viability over time, and E coli did not exhibit a growth relationship with surfaces or fluids. Persistence and adaptability capacity of potential pathogens in fomites and surfaces exposed to the patient are important for guidance, planning, and outlining of protocols for microorganism dissemination control and prevention in the health care environment.
ISSN:0196-6553
1527-3296
DOI:10.1016/j.ajic.2015.09.033