Effect of Endothelial Cells on Angiogenic Properties of Multipotent Stromal Cells from the Umbilical Cord during Angiogenesis Modeling in the Basement Membrane Matrix

Short-term cell culturing on basement membrane matrix is a common and very convenient in vitro model of angiogenesis. We studied the possibility of interaction of multipotent stromal cells from the umbilical cord and Ea.hy926 endothelial cells on this model at the early and late periods of the exper...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2016-02, Vol.160 (4), p.575-582
Main Authors: Arutyunyan, I. V., Fatkhudinov, T. H., El’chaninov, A. V., Makarov, A. V., Kananykhina, E. Yu, Usman, N. Yu, Raimova, E. Sh, Goldshtein, D. V., Bol’shakova, G. B.
Format: Article
Language:English
Subjects:
Analysis
Basement Membrane - cytology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Differentiation
Cell Line
Coculture Techniques
Endothelium
Human Umbilical Vein Endothelial Cells - cytology
Humans
Internal Medicine
Laboratory Medicine
Mesenchymal Stromal Cells - cytology
Neovascularization, Physiologic - physiology
Pathology
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Umbilical Cord - cytology
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Summary:Short-term cell culturing on basement membrane matrix is a common and very convenient in vitro model of angiogenesis. We studied the possibility of interaction of multipotent stromal cells from the umbilical cord and Ea.hy926 endothelial cells on this model at the early and late periods of the experiment. Multipotent stromal cells alone and in combination with endothelial cells formed an unstable tubular network. Clusters formed after its disassembling later became the sprouting centers in co-culture of the two cell types, but not in pure culture of multipotent stromal cells. Multipotent stromal cells with CD31+ phenotype constitute the structural basis of newly formed stable 3D capillary-like network. Prolongation of the time of culturing and combination of the two in vitro models of angiogenesis (tubulogenesis and sprouting) allowed more complete assessment of the angiogenic potential of multipotent stromal cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-016-3221-9