Transcriptome-Derived Microsatellite Markers for Dioon (Zamiaceae) Cycad Species

Premise of the study: Dioon (Zamiaceae) is an endangered North American cycad genus of evolutionary and ornamental value. We designed and validated a set of microsatellite markers from D. edule that can be used for population-level and conservation studies, and that transferred successfully to D. an...

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Bibliographic Details
Published in:Applications in plant sciences 2016-02, Vol.4 (2), p.n/a
Main Authors: Prado, Alberto, Cervantes-Díaz, Fret, Perez-Zavala, Francisco G, González-Astorga, Jorge, Bede, Jacqueline C, Cibrián-Jaramillo, Angélica
Format: Article
Language:English
Subjects:
Alleles
Conservation
cycads
Deoxyribonucleic acid
Dioon edule
DNA
Endangered & extinct species
Gene expression
Genetic diversity
Genetic markers
Genotypes
Heterozygosity
loci
microsatellite repeats
Microsatellites
ornamental value
Phylogenetics
Plant sciences
Population genetics
Population studies
PRIMER NOTES
Primers
trade
transcriptome
Zamiaceae
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Summary:Premise of the study: Dioon (Zamiaceae) is an endangered North American cycad genus of evolutionary and ornamental value. We designed and validated a set of microsatellite markers from D. edule that can be used for population-level and conservation studies, and that transferred successfully to D. angustifolium. D. spinulosum, and D. holmgrenii. Methods and Results: We tested 50 primers from 80 microsatellite candidate loci in the One KP D. edule transcriptome. Genotypes from 21 loci in 20 D. edule individuals revealed up to 14 alleles per locus and observed heterozygosity from 0.15 to 0.92; one locus was monomorphic. Seven of those 21 loci were polymorphic in D. angustifolium, D. spinulosum, and D. holmgrenii, with up to seven alleles, and an observed heterozygosity up to 0.89. Conclusions: The transcriptome-derived microsatellites generated here will serve as tools to advance population genetic studies and inform conservation strategies of Dioon, including the identification and origin of illegal plants in the cycad trade.
ISSN:2168-0450
2168-0450
DOI:10.3732/apps.1500087