Characterization and cross-species amplification of microsatellite loci in the plant pathogenic fungus Leptosphaeria maculans

Seven polymorphic microsatellite markers suitable for population genetic studies and genetic mapping were developed for Leptosphaeria maculans, a fungal pathogen of canola (Brassica napus). Polymorphism was evaluated using 14 isolates from diverse geographical locations. Each locus had either two or...

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Bibliographic Details
Published in:Molecular ecology notes 2004-09, Vol.4 (3), p.480-481
Main Authors: Hayden, H.L, Wilson, L.M, Cozijnsen, A.J, Howlett, B.J
Format: Article
Language:English
Subjects:
alleles
alleles per locus
blackleg
DNA primers
genetic markers
Leptosphaeria
Leptosphaeria biglobosa
Leptosphaeria maculans
loci
microsatellite
microsatellite repeats
molecular sequence data
nucleotide sequences
Phoma
plant pathogenic fungi
polymerase chain reaction
polymorphic loci
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Summary:Seven polymorphic microsatellite markers suitable for population genetic studies and genetic mapping were developed for Leptosphaeria maculans, a fungal pathogen of canola (Brassica napus). Polymorphism was evaluated using 14 isolates from diverse geographical locations. Each locus had either two or three alleles. Cross‐species amplification was observed for almost all loci in L. biglobosa ‘brassicae’ and L. maculans ‘lepidii’.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2004.00719.x