Sox10-rtTA mouse line for tetracycline-inducible expression of transgenes in neural crest cells and oligodendrocytes

Using gene targeting, we inserted a high‐affinity variant of the reverse tetracycline controlled transactivator (rtTA) into the genomic Sox10 locus. This rtTA transgene faithfully recapitulated Sox10 expression in the emerging neural crest, several of its derivatives, and in oligodendrocytes. It was...

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2004-11, Vol.40 (3), p.171-175
Main Authors: Ludwig, Andreas, Schlierf, Beate, Schardt, Anke, Nave, Klaus-Armin, Wegner, Michael
Format: Article
Language:English
Subjects:
Animals
beta-Galactosidase - metabolism
Cell Line
Crosses, Genetic
enteric nervous system
Female
Gene Expression Regulation, Developmental - drug effects
Gene Targeting
High Mobility Group Proteins - genetics
High Mobility Group Proteins - physiology
high-mobility group
Humans
Integrases - genetics
Kinetics
Male
melanocyte
Metabolic Clearance Rate
Mice
Mice, Transgenic
Neoplasm Proteins - genetics
Neoplasm Proteins - physiology
Neural Crest - metabolism
Oligodendroglia - metabolism
Protein Synthesis Inhibitors - pharmacology
Schwann cell
SOXE Transcription Factors
Sry
Tet-on
Tetracycline - pharmacology
Trans-Activators - analysis
Trans-Activators - genetics
Transcription Factors
Transgenes
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Summary:Using gene targeting, we inserted a high‐affinity variant of the reverse tetracycline controlled transactivator (rtTA) into the genomic Sox10 locus. This rtTA transgene faithfully recapitulated Sox10 expression in the emerging neural crest, several of its derivatives, and in oligodendrocytes. It was furthermore able to induce expression of a tetracycline inducible transgenic reporter gene in a doxycycline‐dependent manner. Induction was fast, with substantial reporter gene expression visible 6 h after the onset of doxycycline treatment. Shut‐off, in contrast, exhibited delayed kinetics, which probably correlated with doxycycline clearance rates. This mouse provides a useful tool for generating tetracycline‐controlled gene expression in neural crest and oligodendrocytes. genesis 40:171–175, 2004. © 2004 Wiley‐Liss, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/gene.20083