Perturbation of nucleo‐cytoplasmic transport affects size of nucleus and nucleolus in human cells

Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30–80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo‐cytoplasmic transport causes...

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Bibliographic Details
Published in:FEBS letters 2016-03, Vol.590 (5), p.631-643
Main Authors: Ganguly, Abira, Bhattacharjee, Chumki, Bhave, Madhura, Kailaje, Vaishali, Jain, Bhawik K., Sengupta, Isha, Rangarajan, Annapoorni, Bhattacharyya, Dibyendu
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
beta Karyopherins - genetics
cancer
Cell Line
Cell Nucleolus - metabolism
Cell Nucleus Size
Cytosol - metabolism
Humans
importin beta
karyoplasmic ratio
nuclear transport
nucleolus size
nucleus size
organelle size
Ran
ran GTP-Binding Protein - genetics
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Summary:Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30–80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo‐cytoplasmic transport causes the KR value to increase by 30–50% in immortalized cell lines. Nucleolus volumes are significantly increased in transformed cell lines and the attenuation of nucleo‐cytoplasmic transport causes a significant increase in the nucleolus volume of immortalized cell lines. A cytosol and nuclear fraction swapping experiment emphasizes the potential role of unknown cytosolic factors in nuclear and nucleolar size regulation.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12077