Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone

3-Nitrobenzanthrone (NBA) is one of the most mutagenic nitroaromatic compounds that has been found recently in diesel exhaust and airborne particles. A [ 32P]-postlabelling analysis was carried out to examine the adducts in DNA from human hepatoma HepG2 cells treated with NBA. Two major and two mino...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2000-10, Vol.470 (2), p.133-139
Main Authors: Kawanishi, Masanobu, Enya, Takeji, Suzuki, Hitomi, Takebe, Hiraku, Matsui, Saburo, Yagi, Takashi
Format: Article
Language:English
Subjects:
3-nitrobenzanthrone
3-Nitrobenznathrone
32P-postlabelling analysis
Biological and medical sciences
Chemical mutagenesis
DAN adduct
Human cell
Medical sciences
Toxicology
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Summary:3-Nitrobenzanthrone (NBA) is one of the most mutagenic nitroaromatic compounds that has been found recently in diesel exhaust and airborne particles. A [ 32P]-postlabelling analysis was carried out to examine the adducts in DNA from human hepatoma HepG2 cells treated with NBA. Two major and two minor adduct spots were obtained in the analysis. The structure of the compound obtained from one of the minor adduct spots was identified to be N-acetyl-3-amino-2-(2′-deoxyguanosin-3′,5′-bisphosphate-8-yl)-benzanthrone, based on identical mobility of the compound with that of synthetic standards in thin-layer chromatography and high performance liquid chromatography. This substance is the identical adduct found in our previous in vitro study. The yet-unidentified major adduct spots may be guanosin- and adenosin-benzanthrone adducts without the N-acetyl group.
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5742(00)00053-3