Cloning, Mapping, and Expression Analysis of a Gene Encoding a Novel Mammalian EGF-Related Protein (SCUBE1)

• Published in: Genomics (San Diego, Calif.) Calif.), 2000-11, Vol.70 (1), p.74-81
• Main Authors: Grimmond, Sean, Larder, Rachel, Van Hateren, Nick, Siggers, Pam, Hulsebos, Theo J.M., Arkell, Ruth, Greenfield, Andy
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Astrocytoma - genetics; Biological and medical sciences; Calcium-Binding Proteins; chromosome 15; chromosome 22; Chromosome Mapping; Chromosomes, Human, Pair 22; Cloning, Molecular; Embryo, Mammalian; Epidermal Growth Factor - genetics; Epidermal Growth Factor - metabolism; epidermal growth factor-related protein; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes. Genome; Humans; Intercellular Signaling Peptides and Proteins; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Scube1 gene; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tissue Distribution
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.2000.6370

The epidermal growth factor (EGF) superfamily comprises a diverse group of proteins that function as secreted signaling molecules, growth factors, and components of the extracellular matrix, many with a role in vertebrate development. We have isolated a novel mammalian gene encoding an EGF-related protein with a CUB (C1s-like) domain that defines a new mammalian gene family. The Scube1 (signal peptide-CUB domain-EGF-related 1) gene was isolated from a developing mouse urogenital ridge cDNA library and is expressed prominently in the developing gonad, nervous system, somites, surface ectoderm, and limb buds. We have mapped Scube1 to mouse chromosome 15 and show that it is orthologous to a human gene in the syntenic region of chromosome 22q13. We discuss the possible functions of this novel gene and its role in heritable disease in light of these data.