Detection and cloning of expressed sequences linked to a target gene

DNA markers tightly linked to a target gene are essential starting points for positional cloning. We combined "differential display of mRNA" and "bulked segregant analysis" in order to detect and clone ten expressed sequences as markers linked to a virus resistance gene in Phaseo...

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Bibliographic Details
Published in:Theoretical and applied genetics 2000-11, Vol.101 (7), p.1109-1113
Main Authors: VALLEJOS, C. E, MALANDRO, J. J, SHEEHY, K, ZIMMERMANN, M. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosomes
Classical genetics, quantitative genetics, hybrids
Cloning
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Genotype & phenotype
Leaves
Phaseolus vulgaris
Polymorphism
Pteridophyta, spermatophyta
Vegetals
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Summary:DNA markers tightly linked to a target gene are essential starting points for positional cloning. We combined "differential display of mRNA" and "bulked segregant analysis" in order to detect and clone ten expressed sequences as markers linked to a virus resistance gene in Phaseolus vulgaris. The combination of these two procedures could be used in lieu of positional cloning, provided polymorphisms detectable by differential display exist in the target gene. Isolation of expressed sequences from specific chromosome regions can also be accomplished by combining these procedures.[PUBLICATION ABSTRACT]
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220051586