New ways of determining structural groups in brown coals and their bioconversion products by FT IR spectroscopy

New methods of determining the structural groups -COOH and -CH2- have been developed. The investigation of carboxyl groups is possible both after derivatization with p-fluorophenacylbromide and by quantitative interpretation of the Fourier transform infrared (FT IR) spectra. There exists a linear re...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2000-11, Vol.54 (5), p.681-685
Main Authors: WEBER, A, TESCH, S, THOMAS, B, SCHMIERS, H
Format: Article
Language:English
Subjects:
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Coal - analysis
Fourier transforms
Fundamental and applied biological sciences. Psychology
Infrared spectroscopy
Magnetic Resonance Spectroscopy
Methods. Procedures. Technologies
NMR
Nuclear magnetic resonance
Others
p-fluorophenacylbromide
Solubility
Spectroscopy, Fourier Transform Infrared
Spectrum analysis
Studies
Trichoderma - metabolism
Various methods and equipments
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Summary:New methods of determining the structural groups -COOH and -CH2- have been developed. The investigation of carboxyl groups is possible both after derivatization with p-fluorophenacylbromide and by quantitative interpretation of the Fourier transform infrared (FT IR) spectra. There exists a linear relationship between the results of these two methods that is generally valid for the analysis of all brown coal components. The maximum extinction coefficient of the symmetric stretching vibration band of the CH2 groups has been determined using model substances. This allows quantification of this structural group directly from the FT IR spectrum. The results agree with the contents of methylene groups as determined by 13C-cross polarization-magic angle spinning nuclear magnetic resonance (13C CPMAS NMR) spectroscopy. Using these methods, the COOH and CH2 groups contained in brown coals of the North Rhine region and in their bioconversion products have been quantified.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530000419