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Wavelength-dependent photooxidation and photoreduction of protochlorophyllide and protochlorophyll in the innermost leaves of cabbage (Brassica oleracea var. capitata L.)
The photoreduction and photooxidation processes of different protochlorophyll(ide) forms were studied in the innermost leaves of cabbage (Brassica oleracea var. capitata L.) under monochromatic irradiations. Room-temperature fluorescence emission spectra were measured from the same leaf spots before...
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Published in: | Photosynthesis research 2016-04, Vol.128 (1), p.73-83 |
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description | The photoreduction and photooxidation processes of different protochlorophyll(ide) forms were studied in the innermost leaves of cabbage (Brassica oleracea var. capitata L.) under monochromatic irradiations. Room-temperature fluorescence emission spectra were measured from the same leaf spots before and after illumination to follow the wavelength dependence of the photochemical reactions. Short-wavelength light of 7 µmol photons m⁻² s⁻¹ (625–630 nm) provoked mainly bleaching, and longer wavelengths (630–640 nm) caused both bleaching and photoreduction, while above 640 nm resulted in basically photoreduction. When bleached leaves were kept in darkness at room temperature, all protochlorophyll(ide) forms regenerated during 72 h. Oxygen-reduced environment decreased the extent of bleaching suggesting the involvement of reactive oxygen species. These results confirm that the short-wavelength, 628 nm absorbing, and 633 nm emitting protochlorophyll(ide) form in etiolated cabbage leaves sensibilizes photooxidation. However, the 628 nm light at low intensities stimulates the photoreduction of the longer wavelength protochlorophyllide forms. Kinetic measurements showed that photoreduction saturates at a low PFD (photon flux density) compared to bleaching, suggesting that the quantum yield of photoreduction is higher than that of bleaching. |
doi_str_mv | 10.1007/s11120-015-0200-3 |
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Room-temperature fluorescence emission spectra were measured from the same leaf spots before and after illumination to follow the wavelength dependence of the photochemical reactions. Short-wavelength light of 7 µmol photons m⁻² s⁻¹ (625–630 nm) provoked mainly bleaching, and longer wavelengths (630–640 nm) caused both bleaching and photoreduction, while above 640 nm resulted in basically photoreduction. When bleached leaves were kept in darkness at room temperature, all protochlorophyll(ide) forms regenerated during 72 h. Oxygen-reduced environment decreased the extent of bleaching suggesting the involvement of reactive oxygen species. These results confirm that the short-wavelength, 628 nm absorbing, and 633 nm emitting protochlorophyll(ide) form in etiolated cabbage leaves sensibilizes photooxidation. However, the 628 nm light at low intensities stimulates the photoreduction of the longer wavelength protochlorophyllide forms. Kinetic measurements showed that photoreduction saturates at a low PFD (photon flux density) compared to bleaching, suggesting that the quantum yield of photoreduction is higher than that of bleaching.</description><identifier>ISSN: 0166-8595</identifier><identifier>EISSN: 1573-5079</identifier><identifier>DOI: 10.1007/s11120-015-0200-3</identifier><identifier>PMID: 26519365</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>ambient temperature ; Biochemistry ; Biomedical and Life Sciences ; bleaching ; Brassica - chemistry ; Brassica - metabolism ; Brassica oleracea var. capitata ; cabbage ; Chlorophyll ; Chlorophyll - analogs & derivatives ; Chlorophyll - metabolism ; Enzymes ; etiolation ; Fluorescence ; leaf spot ; leaves ; Life Sciences ; Light ; lighting ; Original Article ; Oxidation ; Oxidation-Reduction ; Oxygen - metabolism ; Photochemical Processes ; photons ; photooxidation ; Plant Genetics and Genomics ; Plant Leaves - chemistry ; Plant Leaves - metabolism ; Plant Physiology ; Plant Sciences ; protochlorophyll ; Protochlorophyllide - metabolism ; reactive oxygen species ; Sunlight ; Vegetables ; wavelengths</subject><ispartof>Photosynthesis research, 2016-04, Vol.128 (1), p.73-83</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>COPYRIGHT 2016 Springer</rights><rights>Springer Science+Business Media Dordrecht 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-341036c6c8d90a36f27b6d664318dbe0a15152baca09b2c66a773b423e16a6723</citedby><cites>FETCH-LOGICAL-c469t-341036c6c8d90a36f27b6d664318dbe0a15152baca09b2c66a773b423e16a6723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26519365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erdei, Anna Laura</creatorcontrib><creatorcontrib>Kósa, Annamária</creatorcontrib><creatorcontrib>Kovács-Smirová, Lilla</creatorcontrib><creatorcontrib>Böddi, Béla</creatorcontrib><title>Wavelength-dependent photooxidation and photoreduction of protochlorophyllide and protochlorophyll in the innermost leaves of cabbage (Brassica oleracea var. capitata L.)</title><title>Photosynthesis research</title><addtitle>Photosynth Res</addtitle><addtitle>Photosynth Res</addtitle><description>The photoreduction and photooxidation processes of different protochlorophyll(ide) forms were studied in the innermost leaves of cabbage (Brassica oleracea var. capitata L.) under monochromatic irradiations. Room-temperature fluorescence emission spectra were measured from the same leaf spots before and after illumination to follow the wavelength dependence of the photochemical reactions. Short-wavelength light of 7 µmol photons m⁻² s⁻¹ (625–630 nm) provoked mainly bleaching, and longer wavelengths (630–640 nm) caused both bleaching and photoreduction, while above 640 nm resulted in basically photoreduction. When bleached leaves were kept in darkness at room temperature, all protochlorophyll(ide) forms regenerated during 72 h. Oxygen-reduced environment decreased the extent of bleaching suggesting the involvement of reactive oxygen species. These results confirm that the short-wavelength, 628 nm absorbing, and 633 nm emitting protochlorophyll(ide) form in etiolated cabbage leaves sensibilizes photooxidation. However, the 628 nm light at low intensities stimulates the photoreduction of the longer wavelength protochlorophyllide forms. Kinetic measurements showed that photoreduction saturates at a low PFD (photon flux density) compared to bleaching, suggesting that the quantum yield of photoreduction is higher than that of bleaching.</description><subject>ambient temperature</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>bleaching</subject><subject>Brassica - chemistry</subject><subject>Brassica - metabolism</subject><subject>Brassica oleracea var. capitata</subject><subject>cabbage</subject><subject>Chlorophyll</subject><subject>Chlorophyll - analogs & derivatives</subject><subject>Chlorophyll - metabolism</subject><subject>Enzymes</subject><subject>etiolation</subject><subject>Fluorescence</subject><subject>leaf spot</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>Light</subject><subject>lighting</subject><subject>Original Article</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxygen - metabolism</subject><subject>Photochemical Processes</subject><subject>photons</subject><subject>photooxidation</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>protochlorophyll</subject><subject>Protochlorophyllide - metabolism</subject><subject>reactive oxygen species</subject><subject>Sunlight</subject><subject>Vegetables</subject><subject>wavelengths</subject><issn>0166-8595</issn><issn>1573-5079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9ks1u1TAQhSMEoreFB2ADkdi0i1z8EzvJslT8VLoSEqViaU2cSa6rXDvYTtW-Ek9Z36Yg2gXyYqQz3xnPSCfL3lCypoRUHwKllJGCUFEQRkjBn2UrKipeCFI1z7MVoVIWtWjEQXYYwhUhpJaUv8wOmBS04VKsst8_4RpHtEPcFh1OaDu0MZ-2Ljp3YzqIxtkcbLdIHrtZ30uuzyefFL0dnXfT9nYcTYcL-UTPjc3jFlOx6HcuxHzE9GnYz9DQtjBgfvzRQwhGQ-5G9KAR8mvw69SfTIQI-WZ98ip70cMY8PVDPcouP3_6cfa12Hz7cn52uil0KZtY8JISLrXUddcQ4LJnVSs7KUtO665FAlRQwVrQQJqWaSmhqnhbMo5UgqwYP8qOl7npkF8zhqh2JmgcR7Do5qBo4msiBaMJff8EvXKzt2m7e4rWTMg9tV6oAUZUxvYuphPT63BntLPYm6SflqXknAhZJcPJI0NiIt7EAeYQ1PnF98csXVjtXQgeezV5swN_qyhR-5SoJSUqpUTtU6J48rx9WHtud9j9dfyJRQLYAoTUsgP6f-76z9R3i6kHp2DwJqjLC5YySAilXErJ7wA8Z9Hk</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Erdei, Anna Laura</creator><creator>Kósa, Annamária</creator><creator>Kovács-Smirová, Lilla</creator><creator>Böddi, Béla</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20160401</creationdate><title>Wavelength-dependent photooxidation and photoreduction of protochlorophyllide and protochlorophyll in the innermost leaves of cabbage (Brassica oleracea var. capitata L.)</title><author>Erdei, Anna Laura ; Kósa, Annamária ; Kovács-Smirová, Lilla ; Böddi, Béla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-341036c6c8d90a36f27b6d664318dbe0a15152baca09b2c66a773b423e16a6723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>ambient temperature</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>bleaching</topic><topic>Brassica - chemistry</topic><topic>Brassica - metabolism</topic><topic>Brassica oleracea var. capitata</topic><topic>cabbage</topic><topic>Chlorophyll</topic><topic>Chlorophyll - analogs & derivatives</topic><topic>Chlorophyll - metabolism</topic><topic>Enzymes</topic><topic>etiolation</topic><topic>Fluorescence</topic><topic>leaf spot</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>Light</topic><topic>lighting</topic><topic>Original Article</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Oxygen - metabolism</topic><topic>Photochemical Processes</topic><topic>photons</topic><topic>photooxidation</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>protochlorophyll</topic><topic>Protochlorophyllide - metabolism</topic><topic>reactive oxygen species</topic><topic>Sunlight</topic><topic>Vegetables</topic><topic>wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erdei, Anna Laura</creatorcontrib><creatorcontrib>Kósa, Annamária</creatorcontrib><creatorcontrib>Kovács-Smirová, Lilla</creatorcontrib><creatorcontrib>Böddi, Béla</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Science (Gale in Context)</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Photosynthesis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erdei, Anna Laura</au><au>Kósa, Annamária</au><au>Kovács-Smirová, Lilla</au><au>Böddi, Béla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wavelength-dependent photooxidation and photoreduction of protochlorophyllide and protochlorophyll in the innermost leaves of cabbage (Brassica oleracea var. capitata L.)</atitle><jtitle>Photosynthesis research</jtitle><stitle>Photosynth Res</stitle><addtitle>Photosynth Res</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>128</volume><issue>1</issue><spage>73</spage><epage>83</epage><pages>73-83</pages><issn>0166-8595</issn><eissn>1573-5079</eissn><abstract>The photoreduction and photooxidation processes of different protochlorophyll(ide) forms were studied in the innermost leaves of cabbage (Brassica oleracea var. capitata L.) under monochromatic irradiations. Room-temperature fluorescence emission spectra were measured from the same leaf spots before and after illumination to follow the wavelength dependence of the photochemical reactions. Short-wavelength light of 7 µmol photons m⁻² s⁻¹ (625–630 nm) provoked mainly bleaching, and longer wavelengths (630–640 nm) caused both bleaching and photoreduction, while above 640 nm resulted in basically photoreduction. When bleached leaves were kept in darkness at room temperature, all protochlorophyll(ide) forms regenerated during 72 h. Oxygen-reduced environment decreased the extent of bleaching suggesting the involvement of reactive oxygen species. These results confirm that the short-wavelength, 628 nm absorbing, and 633 nm emitting protochlorophyll(ide) form in etiolated cabbage leaves sensibilizes photooxidation. However, the 628 nm light at low intensities stimulates the photoreduction of the longer wavelength protochlorophyllide forms. Kinetic measurements showed that photoreduction saturates at a low PFD (photon flux density) compared to bleaching, suggesting that the quantum yield of photoreduction is higher than that of bleaching.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26519365</pmid><doi>10.1007/s11120-015-0200-3</doi><tpages>11</tpages></addata></record> |
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subjects | ambient temperature Biochemistry Biomedical and Life Sciences bleaching Brassica - chemistry Brassica - metabolism Brassica oleracea var. capitata cabbage Chlorophyll Chlorophyll - analogs & derivatives Chlorophyll - metabolism Enzymes etiolation Fluorescence leaf spot leaves Life Sciences Light lighting Original Article Oxidation Oxidation-Reduction Oxygen - metabolism Photochemical Processes photons photooxidation Plant Genetics and Genomics Plant Leaves - chemistry Plant Leaves - metabolism Plant Physiology Plant Sciences protochlorophyll Protochlorophyllide - metabolism reactive oxygen species Sunlight Vegetables wavelengths |
title | Wavelength-dependent photooxidation and photoreduction of protochlorophyllide and protochlorophyll in the innermost leaves of cabbage (Brassica oleracea var. capitata L.) |
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