Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines

In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vector...

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Bibliographic Details
Published in:Research in veterinary science 2016-02, Vol.104, p.83-85
Main Authors: Gao, Dong-sheng, Li, Xiao-jing, Wan, Wen-yan, Li, Hong-jie, Wang, Xiao-xue, Yang, Xia, Li, Yong-tao, Chang, Hong-tao, Chen, Lu, Wang, Chuan-qing, Zhao, Jun
Format: Article
Language:English
Subjects:
Adenoviridae Infections - immunology
Adenoviridae Infections - veterinary
Adenoviridae Infections - virology
Adenovirus
Adenovirus type 4
Adenovirus Vaccines - immunology
Adenoviruses
Adenoviruses, Human - growth & development
Adenoviruses, Human - immunology
Animals
Antigens
Cell Line
Dendritic cells
Hogs
Human adenovirus
Human immunodeficiency virus
Infections
Investigations
Live vector
Macaca mulatta
Porcine cell lines
Recombinant vaccine
Replication
Simian immunodeficiency virus
Swine
Swine Diseases - immunology
Swine Diseases - virology
Swine influenza virus
Vaccines
Vaccines, Synthetic - immunology
Veterinary medicine
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Summary:In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry. •Replication of human Ad4 vector in swine cell lines was investigated for the first time.•Porcine cell lines tested are permissive for Human Ad4 vaccine strain.•Ad4 may be suitable as a recombinant viral vaccine vector or other application in swine.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2015.12.004