Isolation and characterization of a Far-Eastern strain of tick-borne encephalitis virus in China

•A novel TBEV strain (WH2012) was isolated from northern China and its full-length genome was sequenced.•WH2012 was capable of inducing cytopathic effects in human brain-derived cell lines of three origins.•WH2012 replicated most efficiently in human neuroblastoma cells SK-N-SH.•Intracerebral inocul...

Full description

Saved in:
Bibliographic Details
Published in:Virus research 2016-02, Vol.213, p.6-10
Main Authors: Zhang, Xiaowei, Zheng, Zhenhua, Shu, Bo, Mao, Panyong, Bai, Bingke, Hu, Qinxue, Cui, Zongqiang, Wang, Hanzhong
Format: Article
Language:English
Subjects:
Animals
Cell Line
China
Cluster Analysis
Cytopathogenic Effect, Viral
Disease Models, Animal
Encephalitis
Encephalitis Viruses, Tick-Borne - classification
Encephalitis Viruses, Tick-Borne - genetics
Encephalitis Viruses, Tick-Borne - isolation & purification
Encephalitis, Tick-Borne - pathology
Encephalitis, Tick-Borne - virology
Genome, Viral
Growth curve
Human brain-derived cell lines
Humans
Mice
Neurons - virology
Paralysis
Phylogeny
RNA, Viral - genetics
Sequence Analysis, DNA
Sequence Homology
Survival Analysis
Tick-borne encephalitis virus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•A novel TBEV strain (WH2012) was isolated from northern China and its full-length genome was sequenced.•WH2012 was capable of inducing cytopathic effects in human brain-derived cell lines of three origins.•WH2012 replicated most efficiently in human neuroblastoma cells SK-N-SH.•Intracerebral inoculation of virus led to complete paralysis in the hindlimbs and 100% mortality in mice. Tick-borne encephalitis virus (TBEV) is a leading cause of human neurological infection in many parts of Europe and Asia. Although several TBEV isolates have been reported, current understanding of the biological characteristics of a Chinese strain is limited. In this study, a Far-Eastern strain of TBEV designated WH2012 was isolated in northern China. Its genome has been sequenced and found to be closely related to other Chinese TBEV isolates. Human cell lines of neural origin exposed to WH2012 showed cytopathic effects and WH2012 replicated most efficiently in human neuroblastoma cells SK-N-SH. In addition, WH2012 possessed a pathogenic potential in the mouse model, characterized by inducing a complete paralysis in the hindlimbs with a fatal outcome. We herein describe the first data regarding biological properties of TBEV from China. This study may help future research on pathogenic mechanisms of the neurological disease induced by TBEV infection in China.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2015.11.006