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Proteomic response of the phytopathogen Phyllosticta citricarpa to antimicrobial volatile organic compounds from Saccharomyces cerevisiae
Volatile organic compounds (VOCs) released by Saccharomyces cerevisiae inhibit plant pathogens, including the filamentous fungus Phyllosticta citricarpa, causal agent of citrus black spot. VOCs mediate relevant interactions between organisms in nature, and antimicrobial VOCs are promising, environme...
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Published in: | Microbiological research 2016-02, Vol.183, p.1-7 |
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description | Volatile organic compounds (VOCs) released by Saccharomyces cerevisiae inhibit plant pathogens, including the filamentous fungus Phyllosticta citricarpa, causal agent of citrus black spot. VOCs mediate relevant interactions between organisms in nature, and antimicrobial VOCs are promising, environmentally safer fumigants to control phytopathogens. As the mechanisms by which VOCs inhibit microorganisms are not well characterized, we evaluated the proteomic response in P. citricarpa after exposure for 12h to a reconstituted mixture of VOCs (alcohols and esters) originally identified in S. cerevisiae. Total protein was extracted and separated by 2D-PAGE, and differentially expressed proteins were identified by LC–MS/MS. About 600 proteins were detected, of which 29 were downregulated and 11 were upregulated. These proteins are involved in metabolism, genetic information processing, cellular processes, and transport. Enzymes related to energy-generating pathways, particularly glycolysis and the tricarboxylic acid cycle, were the most strongly affected. Thus, the data indicate that antimicrobial VOCs interfere with essential metabolic pathways in P. citricarpa to prevent fungal growth. |
doi_str_mv | 10.1016/j.micres.2015.11.002 |
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VOCs mediate relevant interactions between organisms in nature, and antimicrobial VOCs are promising, environmentally safer fumigants to control phytopathogens. As the mechanisms by which VOCs inhibit microorganisms are not well characterized, we evaluated the proteomic response in P. citricarpa after exposure for 12h to a reconstituted mixture of VOCs (alcohols and esters) originally identified in S. cerevisiae. Total protein was extracted and separated by 2D-PAGE, and differentially expressed proteins were identified by LC–MS/MS. About 600 proteins were detected, of which 29 were downregulated and 11 were upregulated. These proteins are involved in metabolism, genetic information processing, cellular processes, and transport. Enzymes related to energy-generating pathways, particularly glycolysis and the tricarboxylic acid cycle, were the most strongly affected. 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subjects | Amino Acids - metabolism Anti-Infective Agents - pharmacology Antifungal Antifungal Agents - chemistry Antifungal Agents - metabolism Antifungal Agents - pharmacology Ascomycota - drug effects Ascomycota - genetics Ascomycota - metabolism Citric Acid Cycle - drug effects Citrus Citrus - microbiology Electrophoresis, Gel, Two-Dimensional Fungal Proteins - drug effects Fungal Proteins - isolation & purification Fungal Proteins - metabolism Glycolysis - drug effects Guignardia Mechanism of action Nucleotides - metabolism Phyllosticta Plant Diseases - microbiology Postharvest Proteomics Proton-Translocating ATPases - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Tandem Mass Spectrometry Volatile Organic Compounds - chemistry Volatile Organic Compounds - metabolism Volatile Organic Compounds - pharmacology Volatiles |
title | Proteomic response of the phytopathogen Phyllosticta citricarpa to antimicrobial volatile organic compounds from Saccharomyces cerevisiae |
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