Assessment of litter prevalence of Mycoplasma hyopneumoniae in preweaned piglets utilizing an antemortem tracheobronchial mucus collection technique and a real-time polymerase chain reaction assay

The swine industry currently lacks validated antemortem methods of detecting baseline herd prevalence of Mycoplasma hyopneumoniae. The focus of our study was to evaluate alternative antemortem detection techniques and to determine baseline litter prevalence in preweaned pig populations utilizing the...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2015-09, Vol.27 (5), p.606-610
Main Authors: Vangroenweghe, Frédéric, Karriker, Locke, Main, Rodger, Christianson, Eric, Marsteller, Thomas, Hammen, Kristin, Bates, Jessica, Thomas, Paul, Ellingson, Josh, Harmon, Karen, Abate, Sarah, Crawford, Kimberly
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Bronchi - microbiology
Iowa - epidemiology
Mycoplasma hyopneumoniae
Mycoplasma hyopneumoniae - isolation & purification
Pneumonia of Swine, Mycoplasmal - epidemiology
Pneumonia of Swine, Mycoplasmal - microbiology
Prevalence
Real-Time Polymerase Chain Reaction - veterinary
Specimen Handling - veterinary
Swine
Trachea - microbiology
Weaning
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Summary:The swine industry currently lacks validated antemortem methods of detecting baseline herd prevalence of Mycoplasma hyopneumoniae. The focus of our study was to evaluate alternative antemortem detection techniques and to determine baseline litter prevalence in preweaned pig populations utilizing the selected technique and a real-time polymerase chain reaction (qPCR) assay. Preliminary data was analyzed on weaned piglets with evidence of respiratory disease (n = 32). Five sample types (antemortem nasal swab, tracheobronchial mucus, postmortem deep airway swab, bronchoalveolar lavage, and lung tissue) were collected from each pig. Individual samples were tested for M. hyopneumoniae using qPCR. Compared to nasal swabs, tracheobronchial mucus demonstrated higher test sensitivity (P < 0.0001). Tracheobronchial mucus was collected from apparently healthy preweaned piglets (n = 1,759; sow farms 1–3) and preweaned piglets exhibiting signs of respiratory disease (n = 32; sow farm 4), ranging in age from 12 to 25 days. Samples from sow farms 1–3 were pooled into 2 groups of 5 per litter (n = 360 pools from 180 litters), and qPCR was utilized to analyze each pool. A qPCR-positive result, threshold cycle
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638715595062