Immunopotentiator from Pantoea agglomerans Prevents Atopic Dermatitis Induced by Dermatophagoides farinae Extract in NC/Nga Mouse

• Published in: Anticancer research 2015-08, Vol.35 (8), p.4501-4508
• Main Authors: Wakame, Koji, Komatsu, Ken-Ichi, Inagawa, Hiroyuki, Nishizawa, Takashi
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - therapeutic use; Animals; CD4-CD8 Ratio; Cell Adhesion Molecules - blood; Chemokine CCL17 - blood; Dermatitis, Atopic - immunology; Dermatitis, Atopic - prevention & control; Dermatophagoides farinae; Dermatophagoides farinae - immunology; Disease Models, Animal; Immunoglobulin E - blood; Immunoglobulin E - immunology; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - pharmacology; Male; Mice; Mice, Inbred C57BL; Pantoea - immunology; Pantoea - metabolism; Pantoea agglomerans; Skin - immunology; T-Lymphocytes - immunology
• ISSN: 0250-7005; 1791-7530

Pantoea agglomerans LPS (immunopotentiator from Pantoea agglomerans 1: IP-PA1) has been reported to have anti-inflammatory effects in in vitro and in vivo models. The aim of the present study was to investigate the effects of orally-administered IP-PA1 on atopic dermatitis (AD) symptoms induced by Dermatophagoides farinae body extract (DFE) in NC/Nga mice. Using the NC/Nga AD murine model, mice were orally administered 0.1% (High) or 0.01% (Low) water-containing IP-PA1. Skin lesion assessment and blood collection from the caudal vein was performed on days 0, 7, 21 and 31. On day 31, all mice were sacrificed and blood, skin, spleen, as well as intestine samples, were obtained. Assessment score of the skin lesion and serum immunoglobulin E (IgE) level of both IP-PA1 groups were significantly lower than that of the DFE group on days 14 and 21. The serum periostin and thymus and activation-regulated chemokine (TARC) level of IP-PA1-Low group was significantly lower than that of the DFE group on day 31. On histological examination of the skin, hyperplasia of epidermal and dermal layers and infiltration of inflammatory cells were suppressed by IP-PA1 administration. Deposition of periostin was observed in the DFE group skin tissue. Moreover, the CD4(+)/CD8(+) ratio of splenic T-cells increased by IP-PA1 administration. IP-PA1 administration may have an inhibitory effect on AD skin lesions.