Extragenic Suppressors of the nimX2 super(cdc2) Mutation of Aspergillus nidulans Affect Nuclear Division, Septation and Conidiation

The Aspergillus nidulans NIMX super(CDC2) protein kinase has been shown to be required for both the G sub(2)/M and G sub(1)/S transitions, and recent evidence has implicated a role for NIMX super(CDC2) in septation and conidiation. While much is understood of its G sub(2)/M function, little is known...

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Bibliographic Details
Published in:Genetics (Austin) 2000-11, Vol.156 (4), p.1573-1584
Main Authors: McGuire, S L, Roe, D L, Carter, B W, Carter, R L, Grace, S P, Hays, P L, Lang, G A, Mamaril, JLC, McElvaine, A T, Payne, A M, Schrader, MD, Wahrle, SE, Young, C D
Format: Article
Language:English
Subjects:
Aspergillus nidulans
cdc2 gene
hydroxyurea
nimx gene
nimX2 gene
snxA gene
snxB gene
snxC gene
snxD gene
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Summary:The Aspergillus nidulans NIMX super(CDC2) protein kinase has been shown to be required for both the G sub(2)/M and G sub(1)/S transitions, and recent evidence has implicated a role for NIMX super(CDC2) in septation and conidiation. While much is understood of its G sub(2)/M function, little is known about the functions of NIMX super(CDC2) during G sub(1)/S, septation, and conidiophore development. In an attempt to better understand how NIMX super(CDC2) is involved in these processes, we have isolated four extragenic suppressors of the A. nidulans nimX2 super(cdc2) temperature-sensitive mutation. Mutation of these suppressor genes, designated snxA-snxD for suppressor of nmX, affects nuclear division, septation, and conidiation. The cold- sensitive snxA1 mutation leads to arrest of nuclear division during G sub(1) or early S. snxB1 causes hyperseptation in the hyphae and sensitivity to hydroxyurea, while snxC1 causes septation in the conidiophore stalk and aberrant conidiophore structure. snxD1 leads to slight septation defects and hydroxyurea sensitivity. The additional phenotypes that result from the suppressor mutations provide genetic evidence that NIMX super(CDC2) affects septation and conidiation in addition to nuclear division, and cloning and biochemical analysis of these will allow a better understanding of the role of NIMX super(CDC2) in these processes.
ISSN:0016-6731