Molecular Cloning of a Novel Scavenger Receptor for Oxidized Low Density Lipoprotein, SR-PSOX, on Macrophages

Receptor-mediated endocytosis of oxidized low density lipoprotein (OxLDL) by macrophages has been implicated in foam cell transformation in the process of atherogenesis. Although several scavenger receptor molecules, including class A scavenger receptors and CD36, have been identified as OxLDL recep...

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Published in:The Journal of biological chemistry 2000-12, Vol.275 (52), p.40663-40666
Main Authors: Shimaoka, Takeshi, Kume, Noriaki, Minami, Manabu, Hayashida, Kazutaka, Kataoka, Hiroharu, Kita, Toru, Yonehara, Shin
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blotting, Northern
CD36 Antigens
Cell Line
Cloning, Molecular
COS Cells
Endocytosis
Humans
Lipoproteins, LDL - metabolism
Macrophages - chemistry
Membrane Proteins
Molecular Sequence Data
Molecular Weight
Receptors, Immunologic - genetics
Receptors, LDL - genetics
Receptors, Lipoprotein
Receptors, Scavenger
scavenger receptors
Scavenger Receptors, Class A
Scavenger Receptors, Class B
SR-PSOX protein
Tetradecanoylphorbol Acetate - pharmacology
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Summary:Receptor-mediated endocytosis of oxidized low density lipoprotein (OxLDL) by macrophages has been implicated in foam cell transformation in the process of atherogenesis. Although several scavenger receptor molecules, including class A scavenger receptors and CD36, have been identified as OxLDL receptors on macrophages, additional molecules on macrophages may also be involved in the recognition of OxLDL. From a cDNA library of phorbol 12-myristate 13-acetate-stimulated THP-1 cells, we isolated a cDNA encoding a novel protein designated SR-PSOX (scavenger receptor that bindsphosphatidylserine and oxidized lipoprotein), which acts as a receptor for OxLDL. SR-PSOX was a type I membrane protein consisting of 254 amino acids, expression of which was shown on human and murine macrophages with a molecular mass of 30 kDa. SR-PSOX could specifically bind with high affinity, internalize, and degrade OxLDL. The recognition of OxLDL was blocked by polyinosinic acid and dextran sulfate but not by acetylated low density lipoprotein. Taken together, SR-PSOX is a novel class of molecule belonging to the scavenger receptor family, which may play important roles in pathophysiology including atherogenesis.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.C000761200