Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects

• Published in: Sleep & breathing 2016-03, Vol.20 (1), p.271-276
• Main Authors: Minakuchi, Hajime, Sogawa, Chiharu, Miki, Haruna, Hara, Emilio S., Maekawa, Kenji, Sogawa, Norio, Kitayama, Shigeo, Matsuka, Yoshizo, Clark, Glenn Thomas, Kuboki, Takuo
• Format: Article
• Language: English
• Subjects: Adult; Blood Platelets - metabolism; Dentistry; Electromyography; Female; Humans; Internal Medicine; Male; Medicine; Medicine & Public Health; Neurology; Neurology • Original Article; Otorhinolaryngology; Pediatrics; Platelet Count; Pneumology/Respiratory System; Polysomnography; Serotonin; Serotonin - blood; Serotonin Plasma Membrane Transport Proteins - blood; Sleep apnea; Sleep Bruxism - blood; Sleep Bruxism - epidemiology; Statistics as Topic; Young Adult; Young adults
• ISSN: 1520-9512; 1522-1709
• DOI: 10.1007/s11325-015-1281-0

Purpose To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity ( V max ), and an affinity constant ( K m ) were assessed with a [ 3 H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [ 3 H] 5-HT uptake was observed (Spearman’s correlation R 2  = 0.063, p  = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, V max , and K m values ( p  = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.