Effects of light and circadian clock on growth and chlorophyll accumulation of Nannochloropsis gaditana

• Published in: Journal of phycology 2014-06, Vol.50 (3), p.515-525
• Main Authors: Braun, Regina, Farré, Eva M, Schurr, Ulrich, Matsubara, Shizue, Raven, J
• Format: Article
• Language: English
• Subjects: absorbance; blue light; chlorophyll; circadian clock; circadian rhythm; clock entrainment; feedstocks; fluorescence; genome; growth; light-dark; lighting; Marine; microalgae; N annochloropsis; Nannochloropsis; Nannochloropsis gaditana; nucleotide sequences; photobioreactor; photoreceptors; photosystem II; red light
• ISSN: 0022-3646; 1529-8817
• DOI: 10.1111/jpy.12177

Circadian clocks synchronize various physiological, metabolic and developmental processes of organisms with specific phases of recurring changes in their environment (e.g. day and night or seasons). Here, we investigated whether the circadian clock plays a role in regulation of growth and chlorophyll (Chl) accumulation in Nannochloropsis gaditana, an oleaginous marine microalga which is considered as a potential feedstock for biofuels and for which a draft genome sequence has been published. Optical density (OD) of N. gaditana culture was monitored at 680 and 735 nm under 12:12 h or 18:6 h light‐dark (LD) cycles and after switching to continuous illumination in photobioreactors. In parallel, Chl fluorescence was measured to assess the quantum yield of photosystem II. Furthermore, to test if red‐ or blue‐light photoreceptors are involved in clock entrainment in N. gaditana, some of the experiments were conducted by using only red or blue light. Growth and Chl accumulation were confined to light periods in the LD cycles, increasing more strongly in the first half than in the second half of the light periods. After switching to continuous light, rhythmic oscillations continued (especially for OD₆₈₀) at least in the first 24 h, with a 50% decrease in the capacity to grow and accumulate Chl during the first subjective night. Pronounced free‐running oscillations were induced by blue light, but not by red light. In contrast, the photosystem II quantum yield was determined by light conditions. The results indicate interactions between circadian and light regulation of growth and Chl accumulation in N. gaditana.