Mangiferin protect myocardial insults through modulation of MAPK/TGF-β pathways

• Published in: European journal of pharmacology 2016-04, Vol.776, p.34-43
• Main Authors: Suchal, Kapil, Malik, Salma, Gamad, Nanda, Malhotra, Rajiv Kumar, Goyal, Sameer N., Ojha, Shreesh, Kumari, Santosh, Bhatia, Jagriti, Arya, Dharamvir Singh
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Cardiotonic Agents - pharmacology; Gene Expression Regulation - drug effects; Heart - drug effects; Heart - physiopathology; Hemodynamics - drug effects; Inflammation; Interleukin-6 - metabolism; Ischemia-reperfusion injury; Male; Mangiferin; MAP Kinase Signaling System - drug effects; MAPK; Myocardium - metabolism; Myocardium - pathology; Oxidative stress; Rats; Rats, Wistar; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Transforming Growth Factor beta - metabolism; Transforming Growth Factor beta1 - metabolism; Tumor Necrosis Factor-alpha - metabolism; Ventricular Function - drug effects; Xanthones - pharmacology
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2016.02.055

Mangiferin, a xanthone glycoside isolated from leaves of Mangifera indica (Anacardiaceae) is known to modulate many biological targets in inflammation and oxidative stress. The present study was designed to investigate whether mangiferin exerts protection against myocardial ischemia-reperfusion (IR) injury and possible role of Mitogen Activated Protein Kinase (MAPKs) and Transforming Growth Factor-β (TGF-β) pathways in its cardioprotection. Male albino Wistar rats were treated with mangiferin (40mg/kg, i.p.) for 15 days. At the end of the treatment protocol, rats were subjected to IR injury consisting of 45min ischemia followed by 1h reperfusion. IR-control rats caused significant cardiac dysfunction, increased serum cardiac injury markers, lipid peroxidation and a significant decrease in tissue antioxidants as compared to sham group. Histopathological examination of IR rats revealed myocardial necrosis, edema and infiltration of inflammatory cells. However, pretreatment with mangiferin significantly restored myocardial oxidant-antioxidant status, maintained membrane integrity, and attenuated the levels of proinflammatory cytokines, pro-apoptotic proteins and TGF-β. Furthermore, mangiferin significantly reduced the phosphorylation of p38, and JNK and enhanced phosphorylation of ERK1/2. These results suggest that mangiferin protects against myocardial IR injury by modulating MAPK mediated inflammation and apoptosis.