Development of interspecies hybrids to increase ginseng biomass and ginsenoside yield

• Published in: Plant cell reports 2016-04, Vol.35 (4), p.779-790
• Main Authors: Kim, Yu-Jin, Silva, Jeniffer, Zhang, Dabing, Shi, Jianxin, Joo, Sung Chul, Jang, Moon-Gi, Kwon, Woo-Saeng, Yang, Deok-Chun
• Format: Article
• Language: English
• Subjects: Amino acids; Base Sequence; Biological properties; Biomass; Biomedical and Life Sciences; Biotechnology; Cell Biology; Crop Production - methods; crop yield; Crosses, Genetic; fatty acids; Fertility; Flowers - cytology; Genetic Markers; ginsenosides; Ginsenosides - biosynthesis; Herbs; heterosis; Hybridization, Genetic; hybrids; industry; Life Sciences; male fertility; Metabolites; metabolomics; Multiplex Polymerase Chain Reaction; Original Article; Panax - classification; Panax - genetics; Panax - growth & development; Panax - metabolism; Panax ginseng; Phenotype; Plant Biochemistry; Plant Roots - genetics; Plant Sciences; Principal Component Analysis; reciprocal crosses; Roots; Secondary metabolites; Species Specificity
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-015-1920-8

KEY MESSAGE : Interspecific hybrids between Panax ginseng and P. quinquefolius results in hybrid vigor and higher ginsenoside contents. Ginseng is one of the most important herbs with valued pharmaceutical effects contributing mainly by the presence of bioactive ginsenosides in the roots. However, ginseng industry is impeded largely by its biological properties, because ginseng plants are slow-growing perennial herbs with lower yield. To increase the ginseng yield and amounts of ginsenosides, we developed an effective ginseng production system using the F₁ progenies obtained from the interspecific reciprocal cross between two Panax species: P. ginseng and P. quinquefolius. Although hybrid plants show reduced male fertility, F₁ hybrids with the maternal origin either from P. ginseng or P. quinquefolius displayed heterosis; they had larger roots and higher contents of ginsenosides as compared with non-hybrid parental lines. Remarkably, the F₁ hybrids with the maternal origin of P. quinquefolius had much higher ginsenoside contents, especially ginsenoside Re and Rb1, than those with the maternal origin of P. ginseng. Additionally, non-targeted metabolomic profiling revealed a clear increase of a large number of primary and secondary metabolites including fatty acids, amino acids and ginsenosides in hybrid plants. To effectively identify the F₁ hybrids for the large-scale cultivation, we successfully developed a molecular marker detection system for discriminating F₁ reciprocal hybrids. In summary, this work provided a practical system for reciprocal hybrid ginseng production, which would facilitate the ginseng production in the future.