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The Effects of Prone Position Ventilation on Experimental Mild Acute Lung Injury Induced by Intraperitoneal Lipopolysaccharide Injection in Rats
Introduction The benefits of prone position ventilation are well demonstrated in the severe forms of acute respiratory distress syndrome, but not in the milder forms. We investigated the effects of prone position on arterial blood gases, lung inflammation, and histology in an experimental mild acute...
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| Published in: | Lung 2016-04, Vol.194 (2), p.193-199 |
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| creator | Bianchi, Aydra Mendes Almeida Reboredo, Maycon Moura Lucinda, Leda Marília Fonseca Reis, Fernando Fonseca Silva, Manfrinni Vinícius Alves Rabelo, Maria Aparecida Esteves Holanda, Marcelo Alcantara Oliveira, Júlio César Abreu Lorente, José Ángel Pinheiro, Bruno do Valle |
| description | Introduction
The benefits of prone position ventilation are well demonstrated in the severe forms of acute respiratory distress syndrome, but not in the milder forms. We investigated the effects of prone position on arterial blood gases, lung inflammation, and histology in an experimental mild acute lung injury (ALI) model.
Methods
ALI was induced in Wistar rats by intraperitoneal
Escherichia coli
lipopolysaccharide (LPS, 5 mg/kg). After 24 h, the animals with PaO
2
/FIO
2
between 200 and 300 mmHg were randomized into 2 groups: prone position (
n
= 6) and supine position (
n
= 6). Both groups were compared with a control group (
n
= 5) that was ventilated in the supine position. All of the groups were ventilated for 1 h with volume-controlled ventilation mode (tidal volume = 6 ml/kg, respiratory rate = 80 breaths/min, positive end-expiratory pressure = 5 cmH
2
O, inspired oxygen fraction = 1)
Results
Significantly higher lung injury scores were observed in the LPS-supine group compared to the LPS-prone and control groups (0.32 ± 0.03; 0.17 ± 0.03 and 0.13 ± 0.04, respectively) (
p
|
| doi_str_mv | 10.1007/s00408-016-9853-8 |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1774533324</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A446755784</galeid><sourcerecordid>A446755784</sourcerecordid><originalsourceid>FETCH-LOGICAL-c578t-ea6673e28a53d6a640cbee752a404cfe73c09d847e23f2a4eb5a962f3ce28eba3</originalsourceid><addsrcrecordid>eNp1kl-L1DAUxYso7rj6AXyRgCC-dM2_Nu3jsIy6MOIiq68hTW9nMnSSmrTgfAs_src7q-7KSAsJt79zmp6eLHvJ6AWjVL1LlEpa5ZSVeV0VIq8eZQsmBc-ZKujjbEGFZDlH5ix7ltKOUqZKVjzNznhZM85Fsch-3myBrLoO7JhI6Mh1DB7IdUhudMGTb-BH15vbPd6rHwNEt8eh6ckn17dkaacRyHryG3Lld1M84NJOFlrSzNsxmlkxoikq1m4IQ-gPyVi7NdG1MIvw1bO98-SLGdPz7Eln-gQv7tbz7Ov71c3lx3z9-cPV5XKd20JVYw6mLJUAXplCtKUpJbUNgCq4kVTaDpSwtG4rqYCLDofQFKYueScsaqAx4jx7e_QdYvg-QRr13iULfW88hClpppQshBBcIvr6H3QXpujxdLcUrxXn9C-1MT1o57uAH29nU72UslQFnnv2yk9QG_AQTY8pdQ7HD_iLEzxeLeydPSl4c0-wxdjHbQr9NGecHoLsCNoYUorQ6QF_rYkHzaie66WP9dJYLz3XS1eoeXWXxNTsof2j-N0nBPgRSPjIbyDei-q_rr8Ack3Zvw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1774297220</pqid></control><display><type>article</type><title>The Effects of Prone Position Ventilation on Experimental Mild Acute Lung Injury Induced by Intraperitoneal Lipopolysaccharide Injection in Rats</title><source>Springer Nature</source><creator>Bianchi, Aydra Mendes Almeida ; Reboredo, Maycon Moura ; Lucinda, Leda Marília Fonseca ; Reis, Fernando Fonseca ; Silva, Manfrinni Vinícius Alves ; Rabelo, Maria Aparecida Esteves ; Holanda, Marcelo Alcantara ; Oliveira, Júlio César Abreu ; Lorente, José Ángel ; Pinheiro, Bruno do Valle</creator><creatorcontrib>Bianchi, Aydra Mendes Almeida ; Reboredo, Maycon Moura ; Lucinda, Leda Marília Fonseca ; Reis, Fernando Fonseca ; Silva, Manfrinni Vinícius Alves ; Rabelo, Maria Aparecida Esteves ; Holanda, Marcelo Alcantara ; Oliveira, Júlio César Abreu ; Lorente, José Ángel ; Pinheiro, Bruno do Valle</creatorcontrib><description>Introduction
The benefits of prone position ventilation are well demonstrated in the severe forms of acute respiratory distress syndrome, but not in the milder forms. We investigated the effects of prone position on arterial blood gases, lung inflammation, and histology in an experimental mild acute lung injury (ALI) model.
Methods
ALI was induced in Wistar rats by intraperitoneal
Escherichia coli
lipopolysaccharide (LPS, 5 mg/kg). After 24 h, the animals with PaO
2
/FIO
2
between 200 and 300 mmHg were randomized into 2 groups: prone position (
n
= 6) and supine position (
n
= 6). Both groups were compared with a control group (
n
= 5) that was ventilated in the supine position. All of the groups were ventilated for 1 h with volume-controlled ventilation mode (tidal volume = 6 ml/kg, respiratory rate = 80 breaths/min, positive end-expiratory pressure = 5 cmH
2
O, inspired oxygen fraction = 1)
Results
Significantly higher lung injury scores were observed in the LPS-supine group compared to the LPS-prone and control groups (0.32 ± 0.03; 0.17 ± 0.03 and 0.13 ± 0.04, respectively) (
p
< 0.001), mainly due to a higher neutrophil infiltration level in the interstitial space and more proteinaceous debris that filled the airspaces. Similar differences were observed when the gravity-dependent lung regions and non-dependent lung regions were analyzed separately (
p
< 0.05). The BAL neutrophil content was also higher in the LPS-supine group compared to the LPS-prone and control groups (
p
< 0.05). There were no significant differences in the wet/dry ratio and gas exchange levels.
Conclusions
In this experimental extrapulmonary mild ALI model, prone position ventilation for 1 h, when compared with supine position ventilation, was associated with lower lung inflammation and injury.</description><identifier>ISSN: 0341-2040</identifier><identifier>EISSN: 1432-1750</identifier><identifier>DOI: 10.1007/s00408-016-9853-8</identifier><identifier>PMID: 26912235</identifier><identifier>CODEN: LUNGD9</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acute Lung Injury - blood ; Acute Lung Injury - chemically induced ; Acute Lung Injury - pathology ; Acute Lung Injury - prevention & control ; Adult respiratory distress syndrome ; Animals ; Care and treatment ; Complications and side effects ; Disease Models, Animal ; Experiments ; Health aspects ; Influence ; Injections, Intraperitoneal ; Lipopolysaccharides ; Lung - pathology ; Lung - physiopathology ; Lungs ; Male ; Mechanical ventilation ; Medicine ; Medicine & Public Health ; Neutrophil Infiltration ; Pneumology/Respiratory System ; Pneumonia - blood ; Pneumonia - chemically induced ; Pneumonia - pathology ; Pneumonia - prevention & control ; Positive-Pressure Respiration - adverse effects ; Positive-Pressure Respiration - methods ; Prone Position ; Rats, Wistar ; Respiratory Rate ; Rodents ; Supine Position ; Tidal Volume ; Ventilation ; Ventilator-Induced Lung Injury - blood ; Ventilator-Induced Lung Injury - chemically induced ; Ventilator-Induced Lung Injury - pathology ; Ventilator-Induced Lung Injury - prevention & control</subject><ispartof>Lung, 2016-04, Vol.194 (2), p.193-199</ispartof><rights>Springer Science+Business Media New York 2016</rights><rights>COPYRIGHT 2016 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-ea6673e28a53d6a640cbee752a404cfe73c09d847e23f2a4eb5a962f3ce28eba3</citedby><cites>FETCH-LOGICAL-c578t-ea6673e28a53d6a640cbee752a404cfe73c09d847e23f2a4eb5a962f3ce28eba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26912235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bianchi, Aydra Mendes Almeida</creatorcontrib><creatorcontrib>Reboredo, Maycon Moura</creatorcontrib><creatorcontrib>Lucinda, Leda Marília Fonseca</creatorcontrib><creatorcontrib>Reis, Fernando Fonseca</creatorcontrib><creatorcontrib>Silva, Manfrinni Vinícius Alves</creatorcontrib><creatorcontrib>Rabelo, Maria Aparecida Esteves</creatorcontrib><creatorcontrib>Holanda, Marcelo Alcantara</creatorcontrib><creatorcontrib>Oliveira, Júlio César Abreu</creatorcontrib><creatorcontrib>Lorente, José Ángel</creatorcontrib><creatorcontrib>Pinheiro, Bruno do Valle</creatorcontrib><title>The Effects of Prone Position Ventilation on Experimental Mild Acute Lung Injury Induced by Intraperitoneal Lipopolysaccharide Injection in Rats</title><title>Lung</title><addtitle>Lung</addtitle><addtitle>Lung</addtitle><description>Introduction
The benefits of prone position ventilation are well demonstrated in the severe forms of acute respiratory distress syndrome, but not in the milder forms. We investigated the effects of prone position on arterial blood gases, lung inflammation, and histology in an experimental mild acute lung injury (ALI) model.
Methods
ALI was induced in Wistar rats by intraperitoneal
Escherichia coli
lipopolysaccharide (LPS, 5 mg/kg). After 24 h, the animals with PaO
2
/FIO
2
between 200 and 300 mmHg were randomized into 2 groups: prone position (
n
= 6) and supine position (
n
= 6). Both groups were compared with a control group (
n
= 5) that was ventilated in the supine position. All of the groups were ventilated for 1 h with volume-controlled ventilation mode (tidal volume = 6 ml/kg, respiratory rate = 80 breaths/min, positive end-expiratory pressure = 5 cmH
2
O, inspired oxygen fraction = 1)
Results
Significantly higher lung injury scores were observed in the LPS-supine group compared to the LPS-prone and control groups (0.32 ± 0.03; 0.17 ± 0.03 and 0.13 ± 0.04, respectively) (
p
< 0.001), mainly due to a higher neutrophil infiltration level in the interstitial space and more proteinaceous debris that filled the airspaces. Similar differences were observed when the gravity-dependent lung regions and non-dependent lung regions were analyzed separately (
p
< 0.05). The BAL neutrophil content was also higher in the LPS-supine group compared to the LPS-prone and control groups (
p
< 0.05). There were no significant differences in the wet/dry ratio and gas exchange levels.
Conclusions
In this experimental extrapulmonary mild ALI model, prone position ventilation for 1 h, when compared with supine position ventilation, was associated with lower lung inflammation and injury.</description><subject>Acute Lung Injury - blood</subject><subject>Acute Lung Injury - chemically induced</subject><subject>Acute Lung Injury - pathology</subject><subject>Acute Lung Injury - prevention & control</subject><subject>Adult respiratory distress syndrome</subject><subject>Animals</subject><subject>Care and treatment</subject><subject>Complications and side effects</subject><subject>Disease Models, Animal</subject><subject>Experiments</subject><subject>Health aspects</subject><subject>Influence</subject><subject>Injections, Intraperitoneal</subject><subject>Lipopolysaccharides</subject><subject>Lung - pathology</subject><subject>Lung - physiopathology</subject><subject>Lungs</subject><subject>Male</subject><subject>Mechanical ventilation</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neutrophil Infiltration</subject><subject>Pneumology/Respiratory System</subject><subject>Pneumonia - blood</subject><subject>Pneumonia - chemically induced</subject><subject>Pneumonia - pathology</subject><subject>Pneumonia - prevention & control</subject><subject>Positive-Pressure Respiration - adverse effects</subject><subject>Positive-Pressure Respiration - methods</subject><subject>Prone Position</subject><subject>Rats, Wistar</subject><subject>Respiratory Rate</subject><subject>Rodents</subject><subject>Supine Position</subject><subject>Tidal Volume</subject><subject>Ventilation</subject><subject>Ventilator-Induced Lung Injury - blood</subject><subject>Ventilator-Induced Lung Injury - chemically induced</subject><subject>Ventilator-Induced Lung Injury - pathology</subject><subject>Ventilator-Induced Lung Injury - prevention & control</subject><issn>0341-2040</issn><issn>1432-1750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kl-L1DAUxYso7rj6AXyRgCC-dM2_Nu3jsIy6MOIiq68hTW9nMnSSmrTgfAs_src7q-7KSAsJt79zmp6eLHvJ6AWjVL1LlEpa5ZSVeV0VIq8eZQsmBc-ZKujjbEGFZDlH5ix7ltKOUqZKVjzNznhZM85Fsch-3myBrLoO7JhI6Mh1DB7IdUhudMGTb-BH15vbPd6rHwNEt8eh6ckn17dkaacRyHryG3Lld1M84NJOFlrSzNsxmlkxoikq1m4IQ-gPyVi7NdG1MIvw1bO98-SLGdPz7Eln-gQv7tbz7Ov71c3lx3z9-cPV5XKd20JVYw6mLJUAXplCtKUpJbUNgCq4kVTaDpSwtG4rqYCLDofQFKYueScsaqAx4jx7e_QdYvg-QRr13iULfW88hClpppQshBBcIvr6H3QXpujxdLcUrxXn9C-1MT1o57uAH29nU72UslQFnnv2yk9QG_AQTY8pdQ7HD_iLEzxeLeydPSl4c0-wxdjHbQr9NGecHoLsCNoYUorQ6QF_rYkHzaie66WP9dJYLz3XS1eoeXWXxNTsof2j-N0nBPgRSPjIbyDei-q_rr8Ack3Zvw</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Bianchi, Aydra Mendes Almeida</creator><creator>Reboredo, Maycon Moura</creator><creator>Lucinda, Leda Marília Fonseca</creator><creator>Reis, Fernando Fonseca</creator><creator>Silva, Manfrinni Vinícius Alves</creator><creator>Rabelo, Maria Aparecida Esteves</creator><creator>Holanda, Marcelo Alcantara</creator><creator>Oliveira, Júlio César Abreu</creator><creator>Lorente, José Ángel</creator><creator>Pinheiro, Bruno do Valle</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20160401</creationdate><title>The Effects of Prone Position Ventilation on Experimental Mild Acute Lung Injury Induced by Intraperitoneal Lipopolysaccharide Injection in Rats</title><author>Bianchi, Aydra Mendes Almeida ; Reboredo, Maycon Moura ; Lucinda, Leda Marília Fonseca ; Reis, Fernando Fonseca ; Silva, Manfrinni Vinícius Alves ; Rabelo, Maria Aparecida Esteves ; Holanda, Marcelo Alcantara ; Oliveira, Júlio César Abreu ; Lorente, José Ángel ; Pinheiro, Bruno do Valle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-ea6673e28a53d6a640cbee752a404cfe73c09d847e23f2a4eb5a962f3ce28eba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acute Lung Injury - blood</topic><topic>Acute Lung Injury - chemically induced</topic><topic>Acute Lung Injury - pathology</topic><topic>Acute Lung Injury - prevention & control</topic><topic>Adult respiratory distress syndrome</topic><topic>Animals</topic><topic>Care and treatment</topic><topic>Complications and side effects</topic><topic>Disease Models, Animal</topic><topic>Experiments</topic><topic>Health aspects</topic><topic>Influence</topic><topic>Injections, Intraperitoneal</topic><topic>Lipopolysaccharides</topic><topic>Lung - pathology</topic><topic>Lung - physiopathology</topic><topic>Lungs</topic><topic>Male</topic><topic>Mechanical ventilation</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neutrophil Infiltration</topic><topic>Pneumology/Respiratory System</topic><topic>Pneumonia - blood</topic><topic>Pneumonia - chemically induced</topic><topic>Pneumonia - pathology</topic><topic>Pneumonia - prevention & control</topic><topic>Positive-Pressure Respiration - adverse effects</topic><topic>Positive-Pressure Respiration - methods</topic><topic>Prone Position</topic><topic>Rats, Wistar</topic><topic>Respiratory Rate</topic><topic>Rodents</topic><topic>Supine Position</topic><topic>Tidal Volume</topic><topic>Ventilation</topic><topic>Ventilator-Induced Lung Injury - blood</topic><topic>Ventilator-Induced Lung Injury - chemically induced</topic><topic>Ventilator-Induced Lung Injury - pathology</topic><topic>Ventilator-Induced Lung Injury - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bianchi, Aydra Mendes Almeida</creatorcontrib><creatorcontrib>Reboredo, Maycon Moura</creatorcontrib><creatorcontrib>Lucinda, Leda Marília Fonseca</creatorcontrib><creatorcontrib>Reis, Fernando Fonseca</creatorcontrib><creatorcontrib>Silva, Manfrinni Vinícius Alves</creatorcontrib><creatorcontrib>Rabelo, Maria Aparecida Esteves</creatorcontrib><creatorcontrib>Holanda, Marcelo Alcantara</creatorcontrib><creatorcontrib>Oliveira, Júlio César Abreu</creatorcontrib><creatorcontrib>Lorente, José Ángel</creatorcontrib><creatorcontrib>Pinheiro, Bruno do Valle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Family Health Database (Proquest)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Lung</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bianchi, Aydra Mendes Almeida</au><au>Reboredo, Maycon Moura</au><au>Lucinda, Leda Marília Fonseca</au><au>Reis, Fernando Fonseca</au><au>Silva, Manfrinni Vinícius Alves</au><au>Rabelo, Maria Aparecida Esteves</au><au>Holanda, Marcelo Alcantara</au><au>Oliveira, Júlio César Abreu</au><au>Lorente, José Ángel</au><au>Pinheiro, Bruno do Valle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effects of Prone Position Ventilation on Experimental Mild Acute Lung Injury Induced by Intraperitoneal Lipopolysaccharide Injection in Rats</atitle><jtitle>Lung</jtitle><stitle>Lung</stitle><addtitle>Lung</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>194</volume><issue>2</issue><spage>193</spage><epage>199</epage><pages>193-199</pages><issn>0341-2040</issn><eissn>1432-1750</eissn><coden>LUNGD9</coden><abstract>Introduction
The benefits of prone position ventilation are well demonstrated in the severe forms of acute respiratory distress syndrome, but not in the milder forms. We investigated the effects of prone position on arterial blood gases, lung inflammation, and histology in an experimental mild acute lung injury (ALI) model.
Methods
ALI was induced in Wistar rats by intraperitoneal
Escherichia coli
lipopolysaccharide (LPS, 5 mg/kg). After 24 h, the animals with PaO
2
/FIO
2
between 200 and 300 mmHg were randomized into 2 groups: prone position (
n
= 6) and supine position (
n
= 6). Both groups were compared with a control group (
n
= 5) that was ventilated in the supine position. All of the groups were ventilated for 1 h with volume-controlled ventilation mode (tidal volume = 6 ml/kg, respiratory rate = 80 breaths/min, positive end-expiratory pressure = 5 cmH
2
O, inspired oxygen fraction = 1)
Results
Significantly higher lung injury scores were observed in the LPS-supine group compared to the LPS-prone and control groups (0.32 ± 0.03; 0.17 ± 0.03 and 0.13 ± 0.04, respectively) (
p
< 0.001), mainly due to a higher neutrophil infiltration level in the interstitial space and more proteinaceous debris that filled the airspaces. Similar differences were observed when the gravity-dependent lung regions and non-dependent lung regions were analyzed separately (
p
< 0.05). The BAL neutrophil content was also higher in the LPS-supine group compared to the LPS-prone and control groups (
p
< 0.05). There were no significant differences in the wet/dry ratio and gas exchange levels.
Conclusions
In this experimental extrapulmonary mild ALI model, prone position ventilation for 1 h, when compared with supine position ventilation, was associated with lower lung inflammation and injury.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26912235</pmid><doi>10.1007/s00408-016-9853-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0341-2040 |
| ispartof | Lung, 2016-04, Vol.194 (2), p.193-199 |
| issn | 0341-2040 1432-1750 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1774533324 |
| source | Springer Nature |
| subjects | Acute Lung Injury - blood Acute Lung Injury - chemically induced Acute Lung Injury - pathology Acute Lung Injury - prevention & control Adult respiratory distress syndrome Animals Care and treatment Complications and side effects Disease Models, Animal Experiments Health aspects Influence Injections, Intraperitoneal Lipopolysaccharides Lung - pathology Lung - physiopathology Lungs Male Mechanical ventilation Medicine Medicine & Public Health Neutrophil Infiltration Pneumology/Respiratory System Pneumonia - blood Pneumonia - chemically induced Pneumonia - pathology Pneumonia - prevention & control Positive-Pressure Respiration - adverse effects Positive-Pressure Respiration - methods Prone Position Rats, Wistar Respiratory Rate Rodents Supine Position Tidal Volume Ventilation Ventilator-Induced Lung Injury - blood Ventilator-Induced Lung Injury - chemically induced Ventilator-Induced Lung Injury - pathology Ventilator-Induced Lung Injury - prevention & control |
| title | The Effects of Prone Position Ventilation on Experimental Mild Acute Lung Injury Induced by Intraperitoneal Lipopolysaccharide Injection in Rats |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A01%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Effects%20of%20Prone%20Position%20Ventilation%20on%20Experimental%20Mild%20Acute%20Lung%20Injury%20Induced%20by%20Intraperitoneal%20Lipopolysaccharide%20Injection%20in%20Rats&rft.jtitle=Lung&rft.au=Bianchi,%20Aydra%20Mendes%20Almeida&rft.date=2016-04-01&rft.volume=194&rft.issue=2&rft.spage=193&rft.epage=199&rft.pages=193-199&rft.issn=0341-2040&rft.eissn=1432-1750&rft.coden=LUNGD9&rft_id=info:doi/10.1007/s00408-016-9853-8&rft_dat=%3Cgale_proqu%3EA446755784%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c578t-ea6673e28a53d6a640cbee752a404cfe73c09d847e23f2a4eb5a962f3ce28eba3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1774297220&rft_id=info:pmid/26912235&rft_galeid=A446755784&rfr_iscdi=true |