Array technology as a tool to monitor exposure of fish to xenoestrogens

A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technolo...

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Bibliographic Details
Published in:Marine environmental research 2002-09, Vol.54 (3-5), p.395-399
Main Authors: Larkin, Patrick, Folmar, Leroy C, Hemmer, Michael J, Poston, Arianna J, Stephen Lee, H, Denslow, Nancy D
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Biomarkers
biomonitoring
Brackish
Cyprinidae - genetics
Cyprinidae - physiology
Cyprinodon variegatus
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Egg Proteins - biosynthesis
Endocrine disruption
Endocrine System - drug effects
Environmental Exposure
Environmental Monitoring
Estrogen
Estrogens, Non-Steroidal - adverse effects
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Genetic Markers
Macroarray
Marine
mRNA expression
Oligonucleotide Array Sequence Analysis
Pisces
Pollution monitoring
Receptors, Estrogen - drug effects
Sheepshead minnow
Techniques
transferrin
Transferrin - biosynthesis
Vitellogenins - biosynthesis
Water Pollutants, Chemical - adverse effects
xenoestrogens
ZP2 protein
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Summary:A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheepshead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin α and β, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to 17 β-estradiol (E2).
ISSN:0141-1136
1879-0291
DOI:10.1016/S0141-1136(02)00172-1