Zinc concentration effect at the organismal, cellular and subcellular levels in the eastern oyster

The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc...

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Bibliographic Details
Published in:Marine environmental research 2002-09, Vol.54 (3-5), p.401-404
Main Authors: Meiller, Jessica C, Bradley, Brian P
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Crassostrea virginica
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on protozoa and invertebrates
Electrophoresis, Gel, Two-Dimensional
Environmental Exposure
Feces - chemistry
Fundamental and applied biological sciences. Psychology
Gills - physiology
Health Status
Hemolymph
Lysosomal destabilization
Lysosomes - pathology
Marine
Ostreidae - physiology
Oyster
Protein Biosynthesis
Protein signature
Tissue Distribution
Water Pollutants - adverse effects
Water Pollutants - pharmacokinetics
Zinc
Zinc - adverse effects
Zinc - pharmacokinetics
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Summary:The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc.
ISSN:0141-1136
1879-0291
DOI:10.1016/S0141-1136(02)00165-4