Intragenic suppressors that restore the splicing and homing activities of the protein encoded by the second intron of the Saccharomyces capensis cyt b gene

The second (bi2) intron of the mitochondrial cyt b gene from Saccharomyces capensis encodes a bifunctional protein which acts both as a maturase, promoting intron splicing, and as a homing-endonuclease, I-ScaI, promoting intron mobility. In this work we isolated and characterized revertants from a r...

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Bibliographic Details
Published in:Current genetics 2000-12, Vol.38 (5), p.276-282
Main Authors: Jamoussi, K, Lazowska, J
Format: Article
Language:English
Subjects:
cyt b gene
Cytochrome b Group - genetics
Cytochrome b Group - metabolism
Genetic Variation
Introns
maturase
Mutagenesis
Mutagenesis, Site-Directed
Open Reading Frames
RNA - genetics
RNA Splicing
RNA, Fungal - genetics
RNA, Mitochondrial
RNA-Directed DNA Polymerase - genetics
Saccharomyces - genetics
Saccharomyces - growth & development
Saccharomyces - metabolism
Saccharomyces capensis
Saccharomyces cerevisiae Proteins
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Summary:The second (bi2) intron of the mitochondrial cyt b gene from Saccharomyces capensis encodes a bifunctional protein which acts both as a maturase, promoting intron splicing, and as a homing-endonuclease, I-ScaI, promoting intron mobility. In this work we isolated and characterized revertants from a respiratory-deficient mutant in which both functions of the protein have been lost. Intragenic revertants resulted mainly from monosubstitutions in the mutated codon and in one case from a distant second site mutation. All novel variants of the S. capensis bi2 intron-encoded protein are competent for the maturase activity but only two of them can partially complement the homing function.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940000159