Protective effect of β-carbolines and other antioxidants on lipid peroxidation due to hydrogen peroxide in rat brain homogenates

Tryptoline and pinoline are two β-carbolines isolated from the nervous system of mammals. We investigated the ability of these compounds to prevent lipid peroxidation induced by hydrogen peroxide in rat brain homogenates. We also compared their effects with other known antioxidants including melaton...

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Bibliographic Details
Published in:Neuroscience letters 2000-11, Vol.294 (1), p.1-4
Main Authors: Garcı́a, J.J, Martı́nez-Balları́n, E, Robinson, M, Allué, J.L, Reiter, R.J, Osuna, C, Acuña-Castroviejo, D
Format: Article
Language:English
Subjects:
Aldehydes - analysis
Aldehydes - metabolism
Animals
Antioxidants - metabolism
Antioxidants - pharmacology
Ascorbic Acid - metabolism
Ascorbic Acid - pharmacology
b-Carbolines
Biological and medical sciences
Brain Chemistry - drug effects
Brain homogenates
Carbolines - metabolism
Carbolines - pharmacology
Cell-Free System - metabolism
Chromans - pharmacology
Dose-Response Relationship, Drug
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Lipid peroxidation
Lipid Peroxidation - drug effects
Male
Malondialdehyde - analysis
Malondialdehyde - metabolism
Medical sciences
Melatonin
Melatonin - metabolism
Melatonin - pharmacology
Neuropharmacology
Neuroprotective agent
Pharmacology. Drug treatments
Pinoline
Rats
Rats, Sprague-Dawley
Tryptoline
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Summary:Tryptoline and pinoline are two β-carbolines isolated from the nervous system of mammals. We investigated the ability of these compounds to prevent lipid peroxidation induced by hydrogen peroxide in rat brain homogenates. We also compared their effects with other known antioxidants including melatonin, trolox and ascorbic acid. Lipid peroxidation was assessed by measuring malonaldehyde (MDA) and 4-hydroxy-alkenals (4-HDA) concentrations in the brain homogenates. Incubation with hydrogen peroxide (5 mM) increased MDA+4-HDA levels, which were totally prevented by tryptoline, pinoline, melatonin and trolox in a concentration-dependent manner. By contrast, higher MDA-4-HDA concentrations compared with control experiments were found after incubation with ascorbic acid, thus reflecting an increase of lipid peroxidation induced by this compound. Although in vivo studies are needed, the data suggest that these β-carbolines may be potential neuroprotective agents because of their antioxidant activities.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(00)01520-2