Novel Biocatalysts by Identification and Design

Enzymes produced from bacteria and eukaryotic organisms are presently being used for a large variety of different biotechnological applications. The rapidly increasing demand for enzymes which are active towards novel and often non-natural substrates has triggered the development of novel molecular...

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Bibliographic Details
Published in:Biocatalysis and biotransformation 2004-03, Vol.22 (2), p.141-146
Main Authors: Eggert, Thorsten, Leggewie, Christian, Puls, Michael, Streit, Wolfgang, van Pouderoyen, Gertie, Dijkstra, Bauke W., Jaeger, Karl-erich
Format: Article
Language:English
Subjects:
Bacillus subtilis lipase
Bioconversions. Hemisynthesis
biodiversity
Biological and medical sciences
Biotechnology
directed evolution
Fundamental and applied biological sciences. Psychology
metagenome
Methods. Procedures. Technologies
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Summary:Enzymes produced from bacteria and eukaryotic organisms are presently being used for a large variety of different biotechnological applications. The rapidly increasing demand for enzymes which are active towards novel and often non-natural substrates has triggered the development of novel molecular biological methods of enzyme isolation and design. The metagenome approach is a cultivation-independent method which allows the direct cloning and expression of environmental DNA thereby providing access to a wealth of so-far unknown biocatalysts. Additionally, newly identified or existing biocatalysts can be further optimized by different methods of directed evolution. Here, the principle of the metagenome approach is outlined and a strategy is presented for the optimization of a bacterial lipase using a combination of rational design and directed evolution.
ISSN:1024-2422
1029-2446
DOI:10.1080/10242420410001710056